楸树叶中抗氧化活性成分的分离和鉴定

doi:10.11707/j.1001-7488.20140915

Abstract

Abstract:

Our previous study found that ethyl acetate extract phase of Catalpa bungei leaves had strong antioxidant activity. In order to further clarify its antioxidant activity, we have searched the components had strong antioxidant ability though silica gel column chromatography combined with DPPH free radical scavenging methods. The antioxidant components were isolated and purified by semi preparative liquid chromatography. With DPPH radical scavenging activity, reducing capacity and ABTS radical scavenging activity ability as investigation index to find the antioxidant components and the physicochemical properties and spectral data were to identify compounds. The results showed that the ethyl acetate extract phase of C.bungei leaves had two compounds: luteolin(1) and apigenin (2). The antioxidant activity of compound 1 was stronger than BHT and compound 2. DPPH radical scavenging activity, reducing power and ABTS radical scavenging activity IC₅₀ values of compound 1 were 23.89, 29.77 and 43.47 μg ·mL^-1 respectively. The antioxidant activity of compound 1 was stronger than compound 2 mainly because of its adjacent structure of two phenolic hydroxyl groups in the flavone compound nucleus of B ring. Luteolin and apigenin isolated from C.bungei leaves for the first time.

Key words: Catalpa bungei, antioxidant, luteolin, apigenin

CLC Number:

S767.9

Xu Hongyu, Zhang Xiaoru, Wang Junhui, Huang Xiaohua, Lei Ming, Dong Juan, Wei Qin. Isolation and Identification of Bioactive Compounds with Antioxidant from Catalpa bungei[J]. Scientia Silvae Sinicae, 2014, 50(9): 112-117.

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Isolation and Identification of Bioactive Compounds with Antioxidant from Catalpa bungei

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