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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (11): 36-43.doi: 10.11707/j.1001-7488.20141105

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Effects of Different Pollens on Ascorbic Acid Related Enzyme Activities in Apple Fruits

Ge Rui, Guo Xiaojing, Zhang Zhen, Wang Rong, Mao Zhiquan, Chen Xuesen, Wu Shujing, Shen Xiang   

  1. State Key Laboratory of Crop Biology College of Horticultural Science and Engineering, Shandong Agricultural University Tai'an 271018
  • Received:2014-04-19 Revised:2014-07-20 Online:2014-11-25 Published:2014-12-04

Abstract:

Three apple (Malus domestica) cultivars including Fuji, Gala and Golden Delicious were pollinated before the blooming period with pollens from different pollination trees. The ascorbic acid (AsA) metabolism relative enzymes, including ascorbate oxygenase (AAO), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), L-galactono-1,4-lactone dehydrogenase (GalLDH), L-galactose dehydrogenase (GalDH), in the fruits were determined every 20 days during fruit development stage. The results showed that AsA contents increased significantly during 40–60 days after pollination. The GalLDH activity of the pollinated fruits was significantly higher than that of the control. The GalDH activity of the pollinated fruits was also higher than that of the control even though the enzyme had relatively low activity during fruit development process. The activity of MDHAR and DHAR in the pollinated fruits was relatively higher than the controls during the fruit development stage, while the activity of AAO and APX in the pollinated fruits was lower than the controls, but there were no significant differences in those enzymes activities between the pollinated fruits and the controls. Besides, the activity of AAO, APX, DHAR, and MDHAR maintained a relatively high level at the early fruit development stage, then declined and finally remained stable at a low level. The study suggests that the increase of the synthetase activity leads to the increase of AsA contents in the pollinated fruit.

Key words: apple, pollination, ascorbic acid, metabolism, enzyme activity

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