美国白蛾SSR反应体系的优化与初步应用

doi:10.11707/j.1001-7488.20100327

Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (3): 173-177.doi: 10.11707/j.1001-7488.20100327

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Optimization and Preliminary Application of SSR Analysis System in Hyphantria cunea

Du Juan¹ ,Nangong Ziyan² ,Zhou Guona¹ ,Gao Baojia¹

1.College of Forestry, Agricultural University of HebeiBaoding 071000；2.College of Plant Protection, Agricultural University of HebeiBaoding 071000

Received:2008-09-09 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

Abstract

Abstract:

An optimization SSR reaction system was established to investigate the genetic diversity of Hyphantria cunea (Drury). A population of Hyphantria cunea in Cangzhou was used to optimize PCR conditions in the SSR system. The results showed that the best condition was 0.40 mmol·L^-1 dNTP, 2.5 mmol·L^-1 Mg²⁺, 0.32 μmol·L^-1 primers, 1U Taq polymerase, 30～60 ng template DNA in 25 μL SSR reaction system and the optimal annealing temperature of primers was 52 ℃. Genetic diversity of five Hyphantria cunea populations was studied with this SSR reaction system, and the result showed the microsatellite primers were high polymorphic and this system was suitable and stable.

Key words: Hyphantria cunea, SSR, analysis system

Du Juan;Nangong Ziyan;Zhou Guona;Gao Baojia. Optimization and Preliminary Application of SSR Analysis System in Hyphantria cunea[J]. Scientia Silvae Sinicae, 2010, 46(3): 173-177.

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Optimization and Preliminary Application of SSR Analysis System in Hyphantria cunea

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