45S rDNA在7种竹子植物染色体上的定位*

doi:10.11707/j.1001-7488.20091207

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (12): 42-45.doi: 10.11707/j.1001-7488.20091207

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Physical Mapping of the 45S rDNA on Metaphase Chromosomes in Seven Bamboo Species by in situ Hybridization

Xu Chuanmei,Lu Jiangjie,Tang Dingqin

Zhejiang Key Laboratory of Modern Silvicultural Technology Zhejiang Forestry University Lin’an 311300

Received:2008-09-19 Revised:1900-01-01 Online:2009-12-25 Published:2009-12-25
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Abstract

Abstract:

Fluorescence in situ hybridization (FISH) was first applied in locating 45S rDNA sequence on metaphase chromosomes of Phyllostachys edulis, Ph. Bambusoides f.lacrima-deae, Pseudosasa amabilis, Sasa fortunei, S. pygmea, S. argenteastriatus and Bambusa oldhamii. The 45S rDNA locus was detected in the secondary chromosomal constriction region for all the bamboo species investigated. Additional 45S rDNA loci were also found in non-satellite chromosomes in a sympodial bamboo species—B. oldhamii.

Key words: FISH, 45S rDNA, secondary constriction regions, bamboo

CLC Number:

Xu Chuanmei;Lu Jiangjie;Tang Dingqin. Physical Mapping of the 45S rDNA on Metaphase Chromosomes in Seven Bamboo Species by in situ Hybridization[J]. Scientia Silvae Sinicae, 2009, 12(12): 42-45.

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Physical Mapping of the 45S rDNA on Metaphase Chromosomes in Seven Bamboo Species by in situ Hybridization

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