荆条丛枝病植原体的分子检测及鉴定*

doi:10.11707/j.1001-7488.20090629

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (6): 162-165.doi: 10.11707/j.1001-7488.20090629

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Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom

Cui Tingtao¹,Pan Yizhan²,Gao Rui¹,Shao Yunhua¹,Li Xiangdong¹,Shi Chengkui¹,Zhao Wenjun³,Zhu Shuifang³

(1. Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University Tai’an 271018; 2. Shangqiu Vocational and Technical College Shangqiu 476100; 3. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine Beijing 100029)

Received:2008-05-12 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

Abstract

Abstract:

Chaste tree (Vitex negundo var. heterophylla) witches’- broom was a new disease found in Mountain Taishan and the neighboring region. Phytoplasmal cells could be easily observed in the phloem sieve elements of diseased plants under electron microscope. The 16S rRNA gene of phytoplasma associated with this disease (CTWB) was amplified with nested-PCR with the universal primer pairs R16mF2/ R16mR1 and R16F2n/ R16R2 An amplified fragment of ca. 1.2 kb was sequenced and subjected to identity and phylogenetic analyses. Phytoplasma CTWB shared identities of more than 99.0% with members of Aster yellows group (16SrI) in 16S rRNA genes. It had the highest identity of 99.8% with phytoplasmas associated with Maize bushy stunt (subgroup 16SrI-B), and clustered into a common branch with phytoplasmas of the subgroup in the phylogenetic tree constructed with 16S rRNA genes. These results indicated that CTWB belonged to subgroup B of Candidatus Phytoplasma asteris (16SrI-B).

Key words: chaste tree, witches&rsquo, - broom, phytoplasma, 16S rRNA gene, nested-PCR

Cui Tingtao;Pan Yizhan;Gao Rui;Shao Yunhua;Li Xiangdong;Shi Chengkui;Zhao Wenjun;Zhu Shuifang . Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom[J]. Scientia Silvae Sinicae, 2009, 12(6): 162-165.

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Molecular Detection and Identification of the Phytoplasma Associated with Chaste Tree Witches’- Broom

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