Abstract:

Effects of methyl methane sulphonate (MMS) on the morphological changes of the seedlings of Paulownia tomentosa × P.fortunei with witches’ broom and the phytoplasma existence were investigated. The treated seedlings were analyzed with SSR method. The results indicated that MMS treatment was able to morphologically eliminate the diseased symptom of the diseased seedlings, but growth of the seedlings decreased if MMS concentration was over 60 mg ·L^-1. Moreover, there still existed the phytoplasma in the infected seedlings after being treated with 20 mg ·L^-1 MMS, and the digestion sites of those DNA from the healthy, diseased and the diseased seedlings treated with 20 and 100 mg ·L^-1 MMS with SSR analysis were all the same.

Key words: Paulownia tomentosa×P. fortunei;witches&rsquo, broom;methyl methane sulphonate(MMS);morphological change;SSR