绣线菊丛枝病病原的分子鉴定

doi:10.11707/j.1001-7488.20071113

Abstract

Abstract:

A specific fragment of ca. 1.2 kb in length was amplified with PCR from spiraea plants showing typical phytoplasma symptom of yellowing, witches' broom and dieback. The fragment was sequenced and subjected to RFLP and phylogenetic analysis. RFLP analysis showed that the phytoplasma associated with spiraea witches' broom belonged to 16Sr I-B subgroup of aster yellows phytoplasma group. This phytoplasma shared the highest homology of 99.6% with severe aster yellows (SAY) in subgroup 16Sr I-B.

Key words: spiraea witches' broom, phytoplasma, 16S rRNA gene, PCR, RFLP

Gao Rui;Li Xiangdong;Wang Jie;Liu Jinliang;Zhu Xiaoping;Zhang Guangmin. Molecular Identification of the Phytoplasma Associated with Spiraea Witches' Broom[J]. Scientia Silvae Sinicae, 2007, 43(11): 72-75.

[1]	Fu Bing, Ye Xia, Wang Huiyu, Chen Peng, Li Jidong, Zheng Xianbo, Tan Bin, Feng Jiancan. Identification of WRKY Transcription Factors in Jujube and Their Responses to ‘Candidatus Phytoplasma ziziphi’ and Salicylic Acid or Methyl Jasmonate Treatments [J]. Scientia Silvae Sinicae, 2018, 54(8): 65-78.
[2]	Wang He, Ren Zhengguang, Pan Yanping, Feng Shukuai, Lin Caili, Chang Enzhong, Yu Shaoshuai, Tian Guozhong. Determination of Individual Jujube Trees against Jujube Witches'-Broom Disease and Screening of Resistant Varieties from the Ancient Individual Jujube Trees Growing in Beijing [J]. Scientia Silvae Sinicae, 2018, 54(8): 124-132.
[3]	Wang Juan, Chen Hui. Identification and Expression of Cold Shock Domain-containing Protein Gene in Dendroctonus armandi [J]. Scientia Silvae Sinicae, 2018, 54(4): 58-66.
[4]	Wang Shengjie, Wang Shengkun, Lin Caili, Yu Shaoshuai, Wang Laifa, Piao Chungen, Guo Minwei, Tian Guozhong. Loop-Mediated Isothermal Amplification Assay for Detection of Five Phytoplasmas Belonging to 16SrⅠ Group Based on Target tuf Gene [J]. Scientia Silvae Sinicae, 2017, 53(8): 54-63.
[5]	Su Shuoshuo, Li Ming, Wu Pengfei, Zhang Ying, Ma Xiangqing. Cloning and Expression Analysis of Phosphate Transporter Gene ClPht1;1 in Cunninghamia lanceolata [J]. Scientia Silvae Sinicae, 2017, 53(5): 33-42.
[6]	Yu Shaoshuai, Li Yong, Ren Zhengguang, Song Chuansheng, Lin Caili, Piao Chungen, Tian Guozhong. Multilocus Sequence Analysis for Revealing Finer Genetic Variation and Phylogenetic Interrelatedness of Phytoplasma Strains in 16SrI Group in China [J]. Scientia Silvae Sinicae, 2017, 53(3): 105-118.
[7]	Zhang Xiaoling, Wang Liuqiang, Sun Pei, Wu Lishuan, Fan Wei, Zhang Jin, Lu Mengzhu, Hu Jianjun. Differences in Pollen Germination and Allergenic Proteins among Different Populus Germplasms [J]. Scientia Silvae Sinicae, 2017, 53(2): 54-64.
[8]	Li Zhen, Wang Liuqiang, Lu Mengzhu. Effects of PtoWOX11/12a Gene from Populus tomentosa on the Growth and Development of Cutting Seedlings in Poplar [J]. Scientia Silvae Sinicae, 2017, 53(11): 69-76.
[9]	Chu Wenyuan, Wang Yujiao, Zhu Dongyue, Chen Zhu, Yan Hanwei, Xiang Yan. Selection of Novel Reference Genes in Poplar under Salt and Drought Stresses [J]. Scientia Silvae Sinicae, 2017, 53(10): 70-79.
[10]	Liu Wenzhe, Niu Mingyue, Li Xiuyun, Lin Erpei, Huang Huahong, Tong Zaikang. The Selection of Reference Genes for Quantitative PCR in Betula luminifera [J]. Scientia Silvae Sinicae, 2016, 52(8): 29-37.
[11]	Zhao Le, Xing Yilin, Li Zhengnan, Guo Jintang, Ran Longxian. Resistance-Labeling and Stability of Jujube Shrunken-Fruit Disease Pathogen Alternaria alternata to Copper Sulfate [J]. Scientia Silvae Sinicae, 2016, 52(7): 53-58.
[12]	Wang Jiaoli, Nan Xiaoning, Ren Zhengzheng, Ming Jie, Tang Guanghui. Diversity of Intestinal Bacteria Communities from Atrijuglans hetaohei (Lepidoptera: Heliodinidae) and Dichocrocis punctiferalis (Lepidoptera: Pyralidae) Larvae Estimated by PCR-DGGE and T-RFLP Analysis [J]. Scientia Silvae Sinicae, 2016, 52(6): 76-85.
[13]	Luo Linlin, Wu Huajun, Lin Tong. Characterization and Expression of Subunit D Gene of ATP Synthase from Monochamus alternatus [J]. Scientia Silvae Sinicae, 2015, 51(7): 60-68.
[14]	Luo Qunfeng, Xu Meng, Feng Yuanheng, Xu Jiajuan, Li Huogen. Cloning and Bioinformatics of Chalcone Synthase Gene (CHS) in Liriodendron tulipifera and Characterization of Its Tissue Expression [J]. Scientia Silvae Sinicae, 2015, 51(5): 37-45.
[15]	Cheng Longping, Hu Haitao, Guo Weidong, Yang Li, Wang Changchun, Yang Ling. Evaluation and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Elaeagnus umbellata [J]. Scientia Silvae Sinicae, 2015, 51(5): 135-144.

Molecular Identification of the Phytoplasma Associated with Spiraea Witches' Broom

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments