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Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (6): 77-82.doi: 10.11707/j.1001-7488.20090613

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Isolation and Identification of the Principal Acaricidal Components Extracted from Mentha piperita

Ren Jianjun1,2,Shi Guanglu3,Wang Jianwen1,Gu Jicheng1,Wang Younian1,2   

  1. (1.College of Plant Science and Technology in Beiing University of Agriculture Beijing 102206；2.Key Laboratory of New Technology of Agricultural Application of Beijng Beijng 102206；3.Department of Biotechnology in Beijing University of Agriculture Beijing 102206)
  • Received:2008-04-02 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

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Abstract:

The objective of this study was to measure the toxicity of active acaricidal substance of Mentha piperita extracts. Leaves of Mentha piperita were extracted using petroleum ether，chloroform and methanol respectively as solvents. Liquidliquid partition technique was used to separate the chemical compounds. The active acaricidal components were seperated by silica gel column chromatography and thin layer chromatography (TLC). The bioactivity was traced by treating Tetranychus cinnabarinus adults and eggs in vitro and testing the acaricide. Finally，the components of active acaricidal substance was identified by using UV photospectrum，HPLC，IR and MS methods. The effect of acaricidal active substance extracted from Mentha piperita against the adults of Tetranychus cinnabarinus was examined. Compared with those crude extracts with chloroform and methanol，the substance extracted using petroleum ether was more effective，with mortality rate of 87.05% against the adult mites and 93.16% against the eggs. The Duncans test showed that the contact toxicity of the crude extracts from different solvents was significantly different from each other (P＜0.05). A liquidliquid partition from petroleum ether extracts indicated that the active acaricidal substance existed in the petroleum ether fraction. The concentrated extracts of petroleum ether was separated into eight fractions，among which the fraction V produced significantly stronger acaricidal activity than other fractions. The mean half lethal concentration (LC-50) was 0.546 4 mg·mL-1 and 95% confidence limit was 0.326 8～0.913 4 mg·mL-1. Purity of the purified compound was 89.16% by HPLC. IR showed that there were four significant peaks at 1 160，1 450，1 380 and 1 700 cm-1. MS detection showed that the molecular mass of the fraction V was 414 with a steroid structure. The spectrum futher showed that the active acaricidal substance isolated from Mentha piperita extracts likely was a βsitosterol.

Key words: Mentha piperita, isolation and identification, acaricidal active substance, Tetranychus cinnabarinus, &beta, sitosterol