杨树水泡型溃疡病菌培养性状、无性型与分子系统学分析

doi:10.11707/j.1001-7488.20140410

Abstract

Abstract:

The cultural characteristics and anamorph morphology of poplar canker fungus isolates collecting from Shaanxi and Gansu were observed, the rDNA-ITS, β-tubulin and EF-1α gene sequences of these fungal isolates were sequenced using universal primer ITS1/ITS4, EF1-728F/EF1-986R and Bt2a/Bt2b respectively, and the phylogenetic trees were constructed after sequence alignment. The results showed that the 62 isolates could be divided into two colonial groups based on the cultural characteristics on PDA medium: groupⅠ showed typical fountain colony, groupⅡ showed flat colony. Acconding to characteristics of conidium and culture, these isolates are identified as Fusicoccum aesculi, that is anamorph of Botryosphaeria dothidea. Meanwhile tested isolates were clustered into two, three and three groups based on neighbor-joining (NJ) analysis of the rDNA-ITS, β-tubulin and EF-1α gene sequence data respectively. The isolates of groupⅠin three NJ trees, included most tested isolates, were clustered together with B. dothidea retrieved from GenBank, which confirmed the morphological identification of these isolates; the isolate Fldh 1 from Long county was classified as one group in the rDNA-ITS gene phylogenetic trees; the isolates Fyxn, Fyrg and Fyxj from Yangling of Shaanxi were all classified as one separate group in the β-tubulin and EF-1α gene phylogenetic trees; three isolates Fxfh, Fxdh 1 and Fxdh2 from Xianyang of Shaanxi were classified as group Ⅲ in the EF-1α gene phylogenetic trees, displaying far away phylogenetic relationship from other isolates of B. dothidea. Overall there was no one-to-one relationship among cultural characteristics, sporulation and molecular phylogeny of the tested isolates.

Key words: poplar, Botryosphaeriaceae, Fusicoccum, ITS, β-tubulin, EF-1α, systematic

CLC Number:

S763.15

Liang Chaoqiong, Cao Zhimin, Chen Min, Zhou Xue. Cultural Characteristics, Anamorph and DNA Phylogeny of Botryosphaeria dothidea Causing Poplar Canker[J]. Scientia Silvae Sinicae, 2014, 50(4): 66-76.

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Cultural Characteristics, Anamorph and DNA Phylogeny of Botryosphaeria dothidea Causing Poplar Canker

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