Abstract:

Abstract： The extractants of semen pharbitidis was investigated for their biological activity against Tetranychus cinnabarinus. Slide dip and Leafresidue methods were used to measure the biological activity. Liquidliquid distribution and the silica gel column were used to separate and purify active components. The results indicated that bioactivity (touch kill，oviposit inhibition，repellency) of petroleum ether extract of semen pharbitidis was higher than that of methanol extract. The adjusted mortalities of female mite and egg were respectively 93.7% and 77.3% with treatment of 2 mg·mL^-1 of the petroleum ether extract for 24 h，and their LC₅₀ was 0.697 9 and 1.303 7 mg·mL^-1. And the treatment caused 82.51% and 94.54% of oviposition inhibition and repellency rate. The petroleum ether extract of semen pharbitidis was further separated and purified. The bioactivity of petroleum ether purified extracts of semen pharbitidis was higher with the adjusted mortalities of female mite and egg were 96.2% and 82.1%，their LC₅₀ was 0.504 2 and 1.101 6mg·mL^-1. The silica gel column was used to separate and purify active components of the crude purified extracts of petroleum ether. A total of 12 bands were seen with TLC analysis. The band 8 appeared the strongest acarocidal activity against Tetranychus cinnabarinus，and the adjusted mortalities of female mite was 96.4% at the concentration of 2 mg·mL^-1， the LC₅₀ was 0.447 7 mg·mL^-1.

Key words: Key words： semen pharbitidis, Tetranychus cinnabarinus, biological activity, separate