牵牛子杀螨活性物质鉴定及其作用机制初探

doi:10.11707/j.1001-7488.20111132

Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (11): 199-203.doi: 10.11707/j.1001-7488.20111132

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A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus

Du Juan¹, Zhao Lei¹, Shi Guanglu¹, Wang Younian¹, Tong Baosheng²

1. Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture ; College of Biotechnology, Beijing University of Agriculture Beijing 102206;2. Inner Mongolia Yongye Biotechnology Co., Ltd. Huhhot 010010

Received:2010-03-30 Revised:2010-12-13 Online:2011-11-25 Published:2011-11-25

Abstract

Abstract:

Fraction 8 of the extractants from semen pharbitidis was identified and its mechanism of biological activity against Tetranychus cinnabarinus was investigated. The main component of the extractant fraction 8 was detected with nuclear magnetic resonance (NMR), gas chromatography and mass spectrum (GC-MS). Influences of the main component on several enzymes activities such as glutathione-S-transferase (GSTs), acetylcholine esterase (AChE) and mono-amine oxidase (MAO), in T. cinnabarinus body were detected by using biochemical method. Results showed that the main component of the extractant fraction 8 was methyl linoleate. The methyl linoleate activated glutathione-S-transferase in T. cinnabarinus body, while it inhibited to various degrees acetylcholine esterase and mono-amine oxidase related to nervous system, and hence caused the death of T. cinnabarinus. Therefore the methyl linoleate has a good prospect developing into new pesticide.

Key words: Tetranychus cinnabarinus, methyl linoleate, glutathione-S-transferase, acetylcholine esterase, mono-amine oxidase, Ca²⁺-ATPase, Na⁺, K⁺-ATPase

CLC Number:

S436.619

Du Juan;Zhao Lei;Shi Guanglu;Wang Younian;Tong Baosheng. A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus[J]. Scientia Silvae Sinicae, 2011, 47(11): 199-203.

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A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus

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