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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (3): 144-150.doi: 10.11707/j.1001-7488.20140321

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Sequence Analysis of Mitochondrial Cytb Gene of Four Geographic Populations of Carpomya vesuviana

Cheng Xiaotian1, Adil Sattar1, Zhang Wei2, Li Xinquan1   

  1. 1. Key Laboratory of Forestry Ecology and Industry Technology in Arid Region, Education Department of Xinjiang College of Forestry and Horticulture, Xinjiang Agricultural University Urumqi 830052;
    2. Xinjiang Entry-Exit Inspection and Quarantine Bureau Urumqi 830083
  • Received:2013-04-15 Revised:2013-09-21 Online:2014-03-25 Published:2014-04-16
  • Contact: 阿地力·沙塔尔

Abstract:

In this study, PCR amplification and sequencing techniques were used to compare and analyze mitochondrial Cytb gene sequences of four geographic populations (KSS, Turpan, HSX and HMS) of Carpomya vesuviana, to explore the genetic relationship among them. 469 bp fragments were obtained.The composition,specific site and base transition/transversion frequency of the partial sequences of Cytb gene were analyzed. The frequencies of codon usage were calculated and the phylogenetic tree was reconstructed. The results showed that KSS population has a relatively far genetic relationship with Turpan population, HSX population and HMS population.

Key words: Carpomya vesuviana, Cytb, phylogenetic tree, genetic relationship

CLC Number: