宁夏枸杞八氢番茄红素合成酶基因的克隆与序列分析

doi:10.11707/j.1001-7488.20060525

Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (5): 138-141.doi: 10.11707/j.1001-7488.20060525

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Cloning and Sequence Analysis of Phytoene Synthase cDNA from Lycium barbarum

Zheng Yangxia^1,2,Ji Jing¹,Wang Gang¹,Yang Wanshen²

1.College of Agriculture and Biotechnology,Tianjin University Tianjin 300072;2.College of Biology and Science, Sichuan Agricultural University Ya'an 625014

Received:2004-11-22 Revised:1900-01-01 Online:2006-05-25 Published:2006-05-25

Abstract

Abstract:

A novel 1.3 kb cDNA was amplified from cDNA library of Lycium barbarum by PCR.This cDNA clone was further inserted into pGEM-T. The sequence analysis showed that the cDNA was 1 292 bp long with an open reading frame of 1 275 bp, and encoded a polypeptide of 425 animo acids. Its cDNA sequence showed 75%～80% identity, and the amino acid sequence shared 70%～80% identity with those of PSY from Lycopersicon esculentum, Capsicum annuum, Arabidopsis thaliana and Zea mays respectively, indicating that the full-length PSY cDNA was isolated from L. barbarum.

Key words: Lycium barbarum, phytoene synthase, cloning

Zheng Yangxia;Ji Jing;Wang Gang;Yang Wanshen. Cloning and Sequence Analysis of Phytoene Synthase cDNA from Lycium barbarum[J]. Scientia Silvae Sinicae, 2006, 42(5): 138-141.

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Cloning and Sequence Analysis of Phytoene Synthase cDNA from Lycium barbarum

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