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Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (12): 80-85.doi: 10.11707/j.1001-7488.20101213

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Influences of Temperatures,pH Values and Inoculum Concentrations on the Adsorption and Ingression of Ralstonia solanacearum to Eucalyptus urophylla× E. grandis Seedling Root

Wang Shenkun1, Wang Jun2, Kang Lihua1, Xu Daping1   

  1. 1. Research Institute of Tropical Forest, Chinese Academy of Forestry Guangzhou 510520;2. College of Forest, Southern China Agricultural University Guangzhou 510642
  • Received:2010-03-03 Revised:2010-09-17 Online:2010-12-25 Published:2010-12-25

Abstract:

This experiment,the influences of temperatures,pH values and inoculum concentrations on the number of adsorption and ingression of Ralstonia solanacearum to Eucalyptus urophylla × E. grandis root. The optimum temperature for R. solanacearum to adsorb to and ingress in the Eucalyptus root was 30 ℃.The more suitable pH value for the adsorption and ingression was 6.0 or 7.0. Number of the adsorption and ingression was very high under both 3.0×108units ·mL-1 and 3.0×109 units ·mL-1 inoculum concentrations. Higher concentration of R. solanacearum was favorable for its adsorption and ingression,but the number of adsorption and ingression did not increase accordingly when the concentration of bacteria was too high. The best conditions for R. solanacearum adsorption and ingression were 30 ℃,pH value 6.0 or 7.0 and 3.0×108 units ·mL-1 bacterial concentration or higher.

Key words: Ralstonia solanacearum, Eucalyptus urophylla×, E. grandis, temperature, pH value, inoculum concentration, adsorption, ingression

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