Abstract:

The lateral young buds from branches of 11 bamboos, like Pleioblastus fortunei, Pl. viridistriatus, Pseudosasa japonica cv. Tsutsumiana, Phyllostachys aureosulcata f. speciosa, Ph. Heteroclada, Hibanobambusa tranquillans, Chusquea culeou,Chimonocalamus delicatus,Drepanostachyum scandens, Chimonobambusa neopurpurea and Bambusa ventricosa cv. Kimmei, were used as explants. The clustered adventitious buds were induced in 20～60 days after inoculation for different species respectively. Subcultures were performed at intervals of 20～40 days by separation of the multiple shoots in groups of three to five and transferred to fresh medium. The rooting rate for monopodial bamboos was 65%～90%, while for the sympodial ones more than 95%. The rooting rate was of great difference among species. The survival rate of the plantlets was about 90%. According to the observation, the most of plantlet from tissue culture can keep the original morphological characters.

Key words: ornamental bamboos, tissue culture, rapid propagation, morphological variation