Abstract:

Based on the appearance of callus growth and behaviour (structure,color etc.)of Larix principis-Rupprechtii embryogenic callus,the physiological and biochemical background in these different kinds of embryogenic and non-embryogenic calli were analyzed.This included as well callus treated by abscisic acid (ABA).The contents of amino acids,saccharides,hydroxybenzene acid,phytohormones as well as different ions were tested.It was indicated that the average content of 18 amino acids of non-embryogenic calli were higher than embryogenic calli.Especially the contents of Arginine,Lysine and Valine in the non-embryogenic calli were 3～4 times higher as in embryogenic callus.The contents of amylose and trisaccharide have shown no different changes between embryogenic and non-embryogenic calli.The contents of amylos and O-phenylenediphthol in embryogenic calli treated by ABA was rising rapidly,18 times as the un-treated control.The correlation between trisaccharide content and different genotypes was very high.The ethylene content observed in the embryogenic callus was lower compared with the non-embryogenic callus.The Co⁺⁺ content of embryogenic callus was higher than in non-embryogenic callus (12 times).The content of NH⁺₄ was almost double and NO^-₃ was 20-fold higher in NEC.The NH⁺₄/NO^-₃ ratio of embryogenic callus is more than 10 times that of the non-embryogenic callus.The above-mentioned changes might explain some physiological and biochemical differences between these two cell types.

Key words: Larix principis-Rupprechtii, Embryogenic cell line, Amino acid, Genotype