中国松树枯梢病菌遗传多态性的RAPD分析

doi:10.11707/j.1001-7488.20000406

Scientia Silvae Sinicae ›› 2000, Vol. 36 ›› Issue (4): 32-38.doi: 10.11707/j.1001-7488.20000406

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THE USE OF RAPD TO DETECT THE GENETIC VARIATION OF SPHAEROPSIS SAPINEA IN CHINA

Wu Xiaoqin,Huang Minren,Yin Tongmin

Nanjing Forestry University Nanjing 210037

Received:1999-11-04 Revised:1900-01-01 Online:2000-07-25 Published:2000-07-25

Abstract

Abstract: Genomic DNA from 55 isolates of Sphaeropsis sapinea collected on 16 pines and other 2 conifers from 13 provinces in China was studied using random amplified polymorphic DNA markers(RAPDs). The seventeen random primers produced 200 scorable DNA fragments. One hundred ninety seven fragments were polymorphic. The dendrogram generated by UPGMA divided all isolates into three groups. Cluster analysis showed that differences between isolates were not closely related to the host species and that there were complex relations between the isolate differences and geographic sources.

Key words: Sphaeropsis sapinea, RAPD marks, Genetic diversity

Wu Xiaoqin;Huang Minren;Yin Tongmin. THE USE OF RAPD TO DETECT THE GENETIC VARIATION OF SPHAEROPSIS SAPINEA IN CHINA[J]. Scientia Silvae Sinicae, 2000, 36(4): 32-38.

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THE USE OF RAPD TO DETECT THE GENETIC VARIATION OF SPHAEROPSIS SAPINEA IN CHINA

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