白蜡窄吉丁成虫对短时高温的生理响应

doi:10.11707/j.1001-7488.LYKX20210691

摘要/Abstract

摘要：

目的: 从生理水平上探究白蜡窄吉丁成虫温度耐受性形成的原因，为揭示昆虫环境抗逆性形成机制提供参考。方法: 以25 ℃为对照，室内设置温度分别为30、33、37和41 ℃的高温条件，分别于4、8和12 h后收集放置于上述不同条件下的白蜡窄吉丁雌雄成虫。测定各处理样品的含水量、含脂量、糖原、葡萄糖、海藻糖和蛋白质含量的变化。结果: 随着温度升高和高温持续时间延长，白蜡窄吉丁雌雄成虫体内水分和脂类含量都大致呈下降趋势。温度越高时，雌雄虫体内糖原含量越高；而葡萄糖和海藻糖含量则先升高后降低，于33~37 ℃时达到最大值。随着雌雄虫经历高温持续时间的延长，其体内糖原、葡萄糖和海藻糖含量都大致呈增长趋势。雌雄虫体内蛋白质含量随温度升高波动性增加，而随高温持续时间延长表现出逐渐下降趋势。白蜡窄吉丁雄虫体内水分、脂类和糖原含量明显高于雌虫，而海藻糖和蛋白质含量与雌虫相比则相对较低。结论: 短时高温条件下，白蜡窄吉丁雌雄成虫体内含脂量、多种糖类和蛋白质含量均在一定程度上发生明显变化，水分和脂类含量受温度及其持续时间影响较小，糖类和蛋白质含量则受影响相对较大。糖类和蛋白质等多种抗逆物质都在白蜡窄吉丁成虫抵御高温胁迫时发挥了重要的调节作用。

关键词: 白蜡窄吉丁, 耐热性, 短时高温胁迫, 生理生化指标

Abstract:

Objective: This study aims to explore the cause of heat tolerance formation in Agrilus planipennis (emerald ash borer, EAB) adults from the physiological level, so as to provide guidance for revealing the formation mechanism of insect resistance to environmental stress. Method: EAB male and female adults were incubated in different high temperature (30, 33, 37 and 41 ℃) and 25 ℃ (served as the control) conditions for 4, 8 and 12 h, respectively, and the insects were collected to measure the water content, fat content, and glycogen, glucose, trehalose, and protein content. Result: With the increase of temperature and the extension of the duration of high temperature, the contents of water and fat in both male and female adults of EAB showed a downward trend. With temperature increasing, the glycogen content was increased, while the contents of glucose and trehalose increased first and then decreased, reaching the highest level at 33?37 ℃. The contents of glycogen, glucose and trehalose in the male and female adults all generally showed an upward trend with increasing time under high temperature. The protein content in the male and female adults increased with fluctuation with the increase of temperature, but gradually decreased with the extension of high temperature duration. The contents of water, fat and glycogen in EAB male adults were significantly higher than those in the female adults, while the contents of trehalose and protein were relatively lower than those in female adults. Conclusion: Under short-time high-temperature conditions, the fat content, and various sugars and protein contents of EAB adults have changed obviously to some extent. The water and fat contents are less affected by temperature and the treatment duration, while the sugar and protein contents are significantly influenced by temperature and the treatment duration. Many stress-resistant substances, such as sugar and protein, play an important role in regulating the resistance of EAB adults against high temperature conditions.

Key words: emerald ash borer, heat tolerance, high-temperature short-time stress, physiological and biochemical indices

中图分类号:

S718.7

党英侨,王小艺,张彦龙,魏可,曹亮明. 白蜡窄吉丁成虫对短时高温的生理响应[J]. 林业科学, 2023, 59(2): 112-120.

Yingqiao Dang,Xiaoyi Wang,Yanlong Zhang,Ke Wei,Liangming Cao. Physiological Responses of Agrilus planipennis Adults to Short-Time High-Temperature Conditions[J]. Scientia Silvae Sinicae, 2023, 59(2): 112-120.

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参考文献 0

	党英侨, 张彦龙, 王小艺, 等白蜡窄吉丁对高温高湿的耐受性. 林业科学, 2021, 57 (3): 189- 194.
	Dang Y Q, Zhang Y L, Wang X Y, et al Tolerability of emerald ash borer to high temperature and humidity. Scientia Silvae Sinicae, 2021, 57 (3): 189- 194.
	鞠瑞亭. 2012. 城市入侵害虫悬铃木方翅网蝽对高温胁迫的耐受性及其生理机制. 上海: 复旦大学.
	Ju R T. 2012. Tolerance to high temperatures of an urban invasive insect species, the sycamore lace bug, Corythucha ciliata and its physiological mechanisms. Shanghai: Fudan University. [in Chinese]
	李　娟, 李　爽, 王冬梅, 等高温胁迫下西伯利亚蝗体内抗逆物质含量变化. 昆虫学报, 2014, 57 (10): 1155- 1161. doi: 10.16380/j.kcxb.2014.10.002
	Li J, Li S, Wang D M, et al Changes in the contents of stress resistant substances in Gomphocerus sibiricus (Orthoptera: Acrididae) under high temperature stress . Acta Entomologica Sinica, 2014, 57 (10): 1155- 1161. doi: 10.16380/j.kcxb.2014.10.002
	李　爽, 王冬梅, 李　娟, 等雌雄意大利蝗耐高温差异及其生理生化响应对策. 应用昆虫学报, 2015, 52 (4): 960- 967. doi: 10.7679/j.issn.2095-1353.2015.115
	Li S, Wang D M, Li J, et al Differences in heat tolerance and physio-biochemical mechanisms between adult female and male Calliptamus italicus (Orthoptera: Acrididae) . Chinese Journal of Applied Entomology, 2015, 52 (4): 960- 967. doi: 10.7679/j.issn.2095-1353.2015.115
	李　越, 李　晶, 沈诗琪, 等短时高温胁迫对中华地鳖蛋白质和氨基酸含量的影响. 环境昆虫学报, 2020, 42 (4): 979- 984. doi: 10.3969/j.issn.1674-0858.2020.04.23
	Li Y, Li J, Shen S Q, et al Effects of short-time high-temperature stress on protein and amino acid content in Eupolyphaga sinensis Walker . Journal of Environmental Entomology, 2020, 42 (4): 979- 984. doi: 10.3969/j.issn.1674-0858.2020.04.23
	林浩宇, 臧振华, 付烈庆, 等刺桐姬小蜂对低温胁迫的耐受性评价. 森林与环境学报, 2017, 37 (3): 379- 384.
	Lin H Y, Zang Z H, Fu L Q, et al Cold tolerance of Quadrastichu erythrinae to low temperature . Journal of Forest and Environment, 2017, 37 (3): 379- 384.
	刘　超. 2016. 短时高温对亚洲玉米螟的影响. 沈阳: 沈阳农业大学.
	Liu C. 2016. The impact of short-term high temperature on the Asian corn borer (Ostrinia furnacalis). Shenyang: Shenyang Agricultural University. [in Chinese]
	王小艺, 杨忠岐, Gould J R, 等白蜡窄吉丁(鞘翅目: 吉丁甲科)的生物防治研究进展. 中国生物防治学报, 2015, 31 (5): 666- 678.
	Wang X Y, Yang Z Q, Gould J R, et al Biological control progress of Agrilus planipennis (Coleoptera: Buprestidae) . Chinese Journal of Biological Control, 2015, 31 (5): 666- 678.
	文茂羽. 2019. BmFoxO在家蚕(Bombyx mori)应对高温胁迫损伤中的功能研究. 重庆: 西南大学.
	Wen M Y. 2019. Functional study of BmFoxO in response to high temperature stress in silkworm (Bombyx mori). Chongqing: Southwest University. [in Chinese]
	袁瑞玲, 杨　珊, 王晓渭, 等桔小实蝇体重和糖原的积累及其飞行消耗. 环境昆虫学报, 2015, 37 (2): 313- 319.
	Yuan R L, Yang S, Wang X W, et al Body weight fluctuations and glycogen reserve and utilization of Bactrocera dorsalis (Hendel) in its flight processes . Journal of Environmental Entomology, 2015, 37 (2): 313- 319.
	张天浩, 惠　琴, 赵义涛, 等短时高温对绿豆象各虫态生长发育的影响. 应用昆虫学报, 2020, 57 (2): 363- 370. doi: 10.7679/j.issn.2095-1353.2020.040
	Zhang T H, Hui Q, Zhao Y T, et al Effects of short-term heat stress on different developmental stages of Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) . Chinese Journal of Applied Entomology, 2020, 57 (2): 363- 370. doi: 10.7679/j.issn.2095-1353.2020.040
	Baranovská E, Knapp M Small-scale spatiotemporal variability in body size of two common carabid beetles. Central European Journal of Biology, 2014, 9 (5): 476- 494.
	Chown S L, Sørensen J G, Terblanche J S Water loss in insects: an environmental change perspective. Journal of Insect Physiology, 2011, 57 (8): 1070- 1084. doi: 10.1016/j.jinsphys.2011.05.004
	Goebel P C, Bumgardner M S, Herms D A, et al Failure to phytosanitize ash firewood infested with emerald ash borer in a small dry kiln using ISPM-15 standards. Journal of Economic Entomology, 2010, 103 (3): 597- 602. doi: 10.1603/EC09264
	González-Tokman D, Córdoba‐Aguilar A, Dáttilo W, et al Insect responses to heat: physiological mechanisms, evolution and ecological implications in a warming world. Biological Reviews, 2020, 95 (3): 802- 821. doi: 10.1111/brv.12588
	Gou Y P, Guo S F, Wang G, et al Effects of short-term heat stress on the growth and development of Bradysia cellarum and Bradysia impatiens . Journal of Applied Entomology, 2020, 144 (4): 315- 321. doi: 10.1111/jen.12733
	Hamedi N, Moharramipour S, Barzegar M Temperature-dependent chemical components accumulation in Hippodamia variegata (Coleoptera: Coccinellidae) during overwintering . Environmental Entomology, 2013, 42 (2): 375- 380. doi: 10.1603/EN12288
	Hubhachen Z, Madden R D, Dillwith J W Influence of rearing temperature on triacylglycerol storage in the pea aphid, Acyrthosiphon pisum . Archives of Insect Biochemistry and Physiology, 2018, 99 (2): e21495. doi: 10.1002/arch.21495
	Jiang J J, Huang L F, Chen H S, et al Identification of reference genes and expression analysis of heat shock protein genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), after exposure to heat stress . Acta Entomologica Sinica, 2015, 58 (5): 479- 486.
	Kleynhans E, Conlong D E, Terblanche J S Direct and indirect effects of development temperature on adult water balance traits of Eldana saccharina (Lepidoptera: Pyralidae) . Journal of Insect Physiology, 2014, 68, 69- 75. doi: 10.1016/j.jinsphys.2014.06.018
	Malik F A, Reddy Y S Effect of high temperature on haemolymph sugar levels in three selected silkworm races. Acta Entomologica Sinica, 2008, 51 (11): 1113- 1120.
	Musolin D L, Selikhovkin A V, Peregudova E Y, et al North-westward expansion of the invasive range of emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) towards the EU: From Moscow to Saint Petersburg . Forests, 2021, 12 (4): 502. doi: 10.3390/f12040502
	Nauen R, Zimmer C T, Vontas J Heterologous expression of insect P450 enzymes that metabolize xenobiotics. Current Opinion in Insect Science, 2021, 43, 78- 84. doi: 10.1016/j.cois.2020.10.011
	Noer N K, Pagter M, Bahrndorff S, et al Impacts of thermal fluctuations on heat tolerance and its metabolomic basis in Arabidopsis thaliana, Drosophila melanogaster, and Orchesella cincta . PLoS ONE, 2020, 15 (10): e0237201. doi: 10.1371/journal.pone.0237201
	Prasantha B D R, Reichmuth C, Adler C, et al Lipid adsorption of diatomaceous earths and increased water permeability in the epicuticle layer of the cowpea weevil Callosobruchus maculatus (F.) and the bean weevil Acanthoscelides obtectus (Say) (Chrysomelidae) . Journal of Stored Products Research, 2015, 64, 36- 41.
	Su Y Z, Li X R, Zhang W, et al. 2021. Dynamics of heat shock protein responses to thermal stress changes after metamorphosis in a lepidopteran insect. Archives of Insect Biochemistry and Physiology, 107(2): e21791.
	Tusong K B, Guo X X, Meng S S, et al Comparative analysis of the transcriptome of the overwintering desert beetle Microdera punctipennis . Cryobiology, 2017, 78, 80- 89. doi: 10.1016/j.cryobiol.2017.06.009
	USDA. 2021. Animal and Plant Health Inspection Service, Emerald Ash Borer. https://www.aphis.usda.gov/aphis/ ourfocus/planthealth/plant-pest-and-disease-programs/pests-and-diseases/emerald-ash-borer.
	van Dooremalen C, Suring W, Ellers J Fatty acid composition and extreme temperature tolerance following exposure to fluctuating temperatures in a soil arthropod. Journal of Insect Physiology, 2011, 57 (9): 1267- 1273. doi: 10.1016/j.jinsphys.2011.05.017
	Wang X, Bergman R D, Mace T. 2010. Heat sterilization of ash (Fraxinus spp.) firewood: Heat-treating options, temperature monitoring and thermal verification. Wood Material Science and Engineering, 5(2): 104–109.
	Zhang S Z, Fu W Y, Li N, et al Antioxidant responses of Propylaea japonica (Coleoptera: Coccinellidae) exposed to high temperature stress . Journal of Insect Physiology, 2015, 73, 47- 52. doi: 10.1016/j.jinsphys.2015.01.004
	Zhang W, Zhao F, Hoffmann A A, et al A single hot event that does not affect survival but decreases reproduction in the diamondback moth, Plutella xylostella . PLoS ONE, 2013, 8 (10): e75923. doi: 10.1371/journal.pone.0075923

固定值 Constant value	比较值 Relative value	雄 Male			雌 Female
固定值 Constant value	比较值 Relative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 297	2.707	0.031	4, 275	8.157	<0.001
8 h	25~41 ℃	4, 297	5.114	0.001	4, 275	1.204	0.309
12 h	25~41 ℃	4, 297	4.446	0.002	4, 275	1.314	0.265
25 ℃	4~12 h	2, 297	0.246	0.782	2, 275	1.068	0.345
30 ℃	4~12 h	2, 297	3.937	0.021	2, 275	1.572	0.210
33 ℃	4~12 h	2, 297	0.378	0.685	2, 275	0.739	0.478
37 ℃	4~12 h	2, 297	9.274	<0.001	2, 275	0.403	0.668
41 ℃	4~12 h	2, 297	0.979	0.377	2, 275	5.590	0.004

固定值 Constant value	比较值 Relative value	雄 Male			雌 Female
固定值 Constant value	比较值 Relative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 200	1.364	0.248	4, 234	16.283	<0.001
8 h	25~41 ℃	4, 200	4.472	0.002	4, 234	3.707	0.006
12 h	25~41 ℃	4, 200	2.129	0.079	4, 234	2.952	0.021
25 ℃	4~12 h	2, 200	1.028	0.360	2, 234	2.815	0.062
30 ℃	4~12 h	2, 200	3.263	0.040	2, 234	1.528	0.219
33 ℃	4~12 h	2, 200	1.451	0.237	2, 234	4.712	0.010
37 ℃	4~12 h	2, 200	2.678	0.071	2, 234	0.260	0.771
41 ℃	4~12 h	2, 200	0.820	0.442	2, 234	1.205	0.302

固定值 Constant value	比较值 Relative value	雄 Male			雌 Female
固定值 Constant value	比较值 Relative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 234	0.984	0.417	4, 252	6.831	<0.001
8 h	25~41 ℃	4, 234	12.481	<0.001	4, 252	0.540	0.706
12 h	25~41 ℃	4, 234	0.869	0.483	4, 252	8.903	<0.001
25 ℃	4~12 h	2, 234	2.515	0.083	2, 252	2.549	0.080
30 ℃	4~12 h	2, 234	11.460	<0.001	2, 252	1.711	0.183
33 ℃	4~12 h	2, 234	5.283	0.006	2, 252	2.723	0.068
37 ℃	4~12 h	2, 234	1.792	0.169	2, 252	1.252	0.288
41 ℃	4~12 h	2, 234	4.552	0.011	2, 252	2.872	0.058

固定值 Constant value	比较值 Comparative value	雄 Male			雌 Female
固定值 Constant value	比较值 Comparative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 246	4.690	0.001	4, 251	2.341	0.056
8 h	25~41 ℃	4, 246	1.028	0.394	4, 251	0.351	0.843
12 h	25~41 ℃	4, 246	0.173	0.952	4, 251	0.109	0.979
25 ℃	4~12 h	2, 246	0.006	0.994	2, 251	2.222	0.111
30 ℃	4~12 h	2, 246	0.028	0.973	2, 251	3.051	0.049
33 ℃	4~12 h	2, 246	1.650	0.194	2, 251	3.361	0.036
37 ℃	4~12 h	2, 246	0.294	0.746	2, 251	0.031	0.970
41 ℃	4~12 h	2, 246	9.541	<0.001	2, 251	2.565	0.079

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