林业科学 ›› 2020, Vol. 56 ›› Issue (2): 40-47.doi: 10.11707/j.1001-7488.20200205
VuongThi Minh Dien,曾健勇,满秀玲*
收稿日期:
2019-04-23
出版日期:
2020-02-25
发布日期:
2020-03-17
通讯作者:
满秀玲
基金资助:
Thi Minh Dien Vuong,Jianyong Zeng,Xiuling Man*
Received:
2019-04-23
Online:
2020-02-25
Published:
2020-03-17
Contact:
Xiuling Man
摘要:
目的: 研究大兴安岭地区樟子松天然林土壤的碳氮含量与水解酶活性的坡位差异和月份动态,探究土壤碳氮含量与水解酶活性的关系,以期为阐明土壤碳氮转化酶学机制及森林土壤可持续利用提供理论依据。方法: 2018年5-10月每月中旬,在大兴安岭漠河地区樟子松天然林坡地设置试验地。分别在试验地上、中、下坡位各设置3块20 m×30 m的樟子松林试验样地,每样地选3个取样点,每取样点清除枯枝落叶层后,记录不同土深(2.5、7.5、15、25 cm)处土壤温度。同时采集每个取样点不同土层(0~5、5~10、10~20和20~30 cm)的土壤样品测定土壤含水率、碳氮含量(有机碳、全氮)及水解酶(脲酶、蛋白酶、蔗糖酶、纤维素酶)活性。结果: 土壤的有机碳及全氮含量随土层加深而降低,随坡位升高而减少,但坡位差异不显著(P>0.05);0~5、5~10、10~20和20~30 cm土层的有机碳含量分别为68.53~80.38、40.28~46.66、15.86~21.08和11.91~13.79 g·kg-1,土壤全氮含量分别为5.34~5.96、2.98~3.68、2.35~2.61和1.54~1.75 g·kg-1;土壤脲酶、蛋白酶、蔗糖酶及纤维素酶活性随土层加深而降低;随坡位降低,土壤脲酶及蔗糖酶活性增高,蛋白酶与纤维素酶活性降低;月动态分析显示,土壤有机碳和全氮含量高峰期均为9月,酶活性高峰期集中在7-8月;相关分析表明,土壤碳氮(有机碳、全氮)含量与水解酶(脲酶、蔗糖酶、纤维素酶、蛋白酶)活性和含水率均极显著正相关(P < 0.01),土壤温度与有机碳含量显著正相关(P>0.05)、与全氮含量显著负相关(P < 0.05)。结论: 大兴安岭地区樟子松天然林土壤碳氮含量与水解酶活性随土层加深而降低,土壤碳氮含量与酶活性的坡位差异不显著(P>0.05),有机碳和全氮含量高峰期为9月,土壤水解酶活性高峰期集中在7-8月。土壤碳氮含量与水解酶活性极显著相关(P < 0.01),土壤温湿度对碳氮含量与酶活性具有显著影响(P < 0.05)。
中图分类号:
VuongThi Minh Dien,曾健勇,满秀玲. 樟子松天然林土壤碳氮含量与水解酶活性坡位差异及月动态[J]. 林业科学, 2020, 56(2): 40-47.
Thi Minh Dien Vuong,Jianyong Zeng,Xiuling Man. Slope Locational Differences and Monthly Dynamics of Soil Carbon and Nitrogen Contents and Hydrolases Activity in the Natural Forest of Pinus sylvestris var. mongolica[J]. Scientia Silvae Sinicae, 2020, 56(2): 40-47.
表1
土壤碳氮含量、水解酶活性、温湿度Pearson相关性①"
指标 Indicators | 有机碳 Organic carbon | 全氮 Total nitrogen | 脲酶 Urease | 蛋白酶 Protease | 蔗糖酶 Surcease | 纤维素酶 Cellulase | 温度 Temperature | 含水率 Moisture |
有机碳Organic carbon | 1 | 0.807** | 0.818** | 0.788** | 0.792** | 0.721** | 0.195 | 0.699** |
全氮Total nitrogen | 0.807** | 1 | 0.604** | 0.481** | 0.490** | 0.394** | -0.280* | 0.384** |
脲酶Urease | 0.818** | 0.604** | 1 | 0.850** | 0.862** | 0.652** | 0.418** | 0.611** |
蛋白酶Protease | 0.788** | 0.481** | 0.850** | 1 | 0.714** | 0.809** | 0.430** | 0.640** |
蔗糖酶Surcease | 0.792** | 0.490** | 0.862** | 0.714** | 1 | 0.571** | 0.498** | 0.700** |
纤维素酶Cellulase | 0.721** | 0.394** | 0.652** | 0.809** | 0.571** | 1 | 0.452** | 0.393** |
温度Temperature | 0.195 | -0.280* | 0.418** | 0.430** | 0.498** | 0.452** | 1 | 0.292* |
含水率Moisture | 0.699** | 0.384** | 0.611** | 0.640** | 0.700** | 0.393** | 0.292* | 1 |
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