秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系——基于nrDNAITS序列分析

doi:10.11707/j.1001-7488.20141207

林业科学 ›› 2014, Vol. 50 ›› Issue (12): 47-55.doi: 10.11707/j.1001-7488.20141207

秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系——基于nrDNAITS序列分析

胡昳恒¹, 党萌¹, 张甜¹, 罗桂春¹, 夏海龙¹, 周惠娟¹, 呼东峰², 贺亮³, 马振华⁴, 赵鹏¹

1. 西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室西安 710069;
2. 陕西省环境监测中心站西安 710054;
3. 西安市环境监测站西安 710054;
4. 国家林业局西北林业调查规划设计院西安 710048

收稿日期:2014-05-20 修回日期:2014-09-01 出版日期:2014-12-25 发布日期:2015-01-08
通讯作者: 赵鹏
基金资助:
西北大学大学生创新创业训练计划项目(2014003);国家基础科学人才培养基金(J1210063);国家自然科学基金项目(31200500,41471038);西部资源生物与现代生物技术教育部重点实验室基金项目(ZS12017);陕西省教育厅科研计划项目(12JK0829);长江学者和创新团队发展计划资助(IRT1174);西北大学科研启动金(PR11055).

Genetic Diversity and Evolutionary Relationship of Juglans regia Wild and Domesticated Populations in Qinling Mountains Based on nrDNA ITS Sequences

Hu Yiheng¹, Dang Meng¹, Zhang Tian¹, Luo Guichun¹, Xia Hailong¹, Zhou Huijuan¹, Hu Dongfeng², He Liang³, Ma Zhenhua⁴, Zhao Peng¹

1. Key Laboratory of Resource Biology and Biotechnology in Western China of Ministry of Education College of Life Sciences, Northwest University Xi'an 710069;
2. Shaanxi Environmental Monitoring Station Xi'an 710054;
3. Xi'an Environmental Monitoring Station Xi'an 710054;
4. Northwest Institute of Forest Inventory, Planning and Design, State Forestry Administration Xi'an 710048

Received:2014-05-20 Revised:2014-09-01 Online:2014-12-25 Published:2015-01-08

摘要/Abstract

摘要：

基于19个自然群体和10个栽培群体共359个个体的核糖体内转录间隔区(nrDNA ITS)序列分析,研究秦岭地区核桃的群体遗传多样性、群体遗传结构及单倍型地理分布.359条ITS序列上共有18个单倍型,表现出较低的遗传多样性(H_d=0.274,π=0.005 5),群体间单倍型遗传多样性差异较大(H_d=0~0.679, π=0~0.017).Network单倍型网络分析表明,单倍型14和单倍型7比较原始,且栽培群体与自然群体之间对古老单倍型H14与H7有着明显的共享现象,基因流较强(N_m=48.62).综合中性检验及失配分析的结果,可推断在近期历史上秦岭地区核桃没有发生过群体扩张事件.AMOVA分析结果显示,秦岭地区自然核桃群体遗传变异主要存在于群体内(53.4%),群体间遗传分化水平较低(G_ST=0.067).单倍型最大简约树和邻接树均可将18个单倍型及所有核桃群体分成两大支.

关键词: 核桃, 秦岭, 群体遗传结构, ITS序列, 基因流

Abstract:

A total of 359 rDNA ITS sequences were used to study the genetic diversity, population genetic structure and the geographical distribution of haplotype patterns of Juglans regia wild and domesticated populations. The results showed that: based on ITS sequences, 18 haplotypes were found in 29 populations. Nueleotide diversity (π) at species level was 0.005 5,and haplotype diversity(H_d)was 0.274. The haplotype diversity was from 0 to 0.679 in different populations. The gene flow (N_m=48.62) was fairly strong between wild and domesticated populations. Haplotype of network showed that haplotype 7 and haplotype 14 were relatively original in this study. Also, haplotype 7 and haplotype 14 were shared among different populations in wild and domesticated populations. The results of Neutrality test and mismatch distribution analysis showed that common walnut trees have not been expanded in recent history. The result of AMOVA depended on ITS showed that genetic variation of J. regia mainly occurred within wild populations, G_ST was 0.067. The maximum likelihood (ML) tree and neighbor-joining (NJ) tree showed all haplotypes and populations were divided into two clusters.

Key words: Juglans regia, Qinling Mountains, population genetic structure, ITS sequence, gene flow

中图分类号:

胡昳恒, 党萌, 张甜, 罗桂春, 夏海龙, 周惠娟, 呼东峰, 贺亮, 马振华, 赵鹏. 秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系——基于nrDNAITS序列分析[J]. 林业科学, 2014, 50(12): 47-55.

Hu Yiheng, Dang Meng, Zhang Tian, Luo Guichun, Xia Hailong, Zhou Huijuan, Hu Dongfeng, He Liang, Ma Zhenhua, Zhao Peng. Genetic Diversity and Evolutionary Relationship of Juglans regia Wild and Domesticated Populations in Qinling Mountains Based on nrDNA ITS Sequences[J]. Scientia Silvae Sinicae, 2014, 50(12): 47-55.

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秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系——基于nrDNAITS序列分析

Genetic Diversity and Evolutionary Relationship of Juglans regia Wild and Domesticated Populations in Qinling Mountains Based on nrDNA ITS Sequences

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