红枝鸡爪槭枝枯病病原鉴定及防治

doi:10.11707/j.1001-7488.20140616

林业科学 ›› 2014, Vol. 50 ›› Issue (6): 125-130.doi: 10.11707/j.1001-7488.20140616

红枝鸡爪槭枝枯病病原鉴定及防治

王志龙¹, 林立¹, 王国良², 祝志勇¹, 徐湾湾³

1. 宁波城市职业技术学院景观生态环境学院宁波市园林植物开发重点实验室宁波 315502;
2. 浙江万里学院生物与环境学院宁波 315100;
3. 余姚第二职业技术学校余姚 315430

收稿日期:2014-02-28 修回日期:2014-04-03 出版日期:2014-06-25 发布日期:2014-07-07
基金资助:
国家星火计划重大项目“浙江四明山区域槭树和樱花产业提升技术集成与示范”(011C11012)；宁波市农业社会发展重大项目“槭树科种质资源库建设与良种高效栽培技术研究”(2011C11012)。

Identification of Pathogen and Control of Acer palmatum ‘Sangokaku’ Branch Blight

Wang Zhilong¹, Lin Li¹, Wang Guoliang², Zhu Zhiyong¹, Xu Wanwan³

1. College of Landscape & Environmental Sciences, Ningbo Vocational Technical School Key Laboratory of Landscape Plant Utility of Ningbo Ningbo 315502;
2. College of Biological & Environmental Sciences, Zhejiang Wanli University Ningbo 315100;
3. Yuyao Second Occupation Technical School Ningbo 315430

Received:2014-02-28 Revised:2014-04-03 Online:2014-06-25 Published:2014-07-07

摘要/Abstract

摘要：

为了明确浙江省红枝鸡爪槭枝枯病的病原种类，在浙江省四明山区域采集红枝鸡爪槭枝枯病样品，以组织分离法进行病原物的分离培养，对分离得到的炭疽病菌落进行纯化和单菌丝段分离后，以形态学为基础，参照Sutton分类系统进行鉴定。结果表明：从病样中共分离到6个炭疽病菌菌株。菌株的分生孢子圆柱形，单孢无色，具1个油球，两端钝圆，大小（18.23~11.76） μm×（4.46~2.94） μm（平均14.79 μm×4.06 μm）；附着孢淡褐色，近圆形或不规则形，边缘平滑，大小为8.67 μm×4.52 μm，与胶孢炭疽病菌相似。按照柯赫氏法则对红枝鸡爪槭植株上的当年生枝进行致病性测定，证实胶孢炭疽菌对红枝鸡爪槭的致病性。将菌株Z92011的rDNA-ITS序列与GenBank 中相关菌株的ITS序列进行同源性比较，与胶孢炭疽病菌的同源性达到100%。确定浙江省红枝鸡爪槭枝枯病的病原菌为胶孢炭疽病菌。试验表明用40%咪鲜胺乳油800倍液喷雾防治效果达92.6%。

关键词: 红枝鸡爪槭, 枝枯病, 病原菌, 胶孢炭疽菌, 防治

Abstract:

To explore the pathogens of branch blight of Acer palmatum ‘Sangokaku’ in Zhejiang province,The infected samples were collected from Siming mountain area of Zhejiang in September 2013. The pathogens were isolated by tissue isolation method from blighted A. palmatum‘Sangokaku’ branch and cultured on standard medium. The obtained colonies were identified as Colletotrichum according to the taxonomic system of Sutton B C after being subcultured and single hyphal fragment. The results showed that the six isolates were obtained from the sampling sites. Their conidia were cylindrical, colorless single spore, with one oil ball, blunt ends, ranged from (18.23-11.76) μm × (4.46-2.94) μm (average 14.79 μm × 4.06 μ m). The appressoria were nearly round or irregular in shape, edge smoothing, pale brown, average 8.67 μm × 4.52 μ m. Conidia and appressoria were similar with Colletotrichum gloeosporioides in shape and size. Pathogenicity tests were performed by strain Z920121. After inoculation, the same branch blight was observed in 23 days on 100% of inoculated branches. DNA was directly extracted from the mycelium of Z920121 grown on PDA for 10 days. The identities of the isolates were confirmed by ITS1-5.8S-ITS2 rDNA sequence (GenBank Accession No. KF787111) analysis that showed 100% sequence similarity to C. gloeosporioides. (Accession No. KC493156.1,HQ645082.1). It was identified that the pathogen of branch blight of A.palmatum ‘Sangokaku’ in Zhejiang province was C. gloeosporioides. Treatment by spraying the plant with 40% EC 800-times diluted prochloraz liquid had a control effect on the disease up to 92.6%.

Key words: Acer palmatum‘Sangokaku’, branch blight, pathogen, Colletotrichum gloeosporioides, control

中图分类号:

S763.1

王志龙, 林立, 王国良, 祝志勇, 徐湾湾. 红枝鸡爪槭枝枯病病原鉴定及防治[J]. 林业科学, 2014, 50(6): 125-130.

Wang Zhilong, Lin Li, Wang Guoliang, Zhu Zhiyong, Xu Wanwan. Identification of Pathogen and Control of Acer palmatum ‘Sangokaku’ Branch Blight[J]. Scientia Silvae Sinicae, 2014, 50(6): 125-130.

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红枝鸡爪槭枝枯病病原鉴定及防治

Identification of Pathogen and Control of Acer palmatum ‘Sangokaku’ Branch Blight

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