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林业科学 ›› 2014, Vol. 50 ›› Issue (4): 129-136.doi: 10.11707/j.1001-7488.20140419

• 研究简报 • 上一篇    下一篇

青杄干旱诱导基因PwWDS1的cDNA分离与表达分析

李长江1, 崔晓燕2, 孙帆1, 张凌云1   

  1. 1. 北京林业大学林学院 森林培育与保护教育部重点实验室 北京 100083;
    2. 吉林大学植物科学学院 长春 130062
  • 收稿日期:2013-06-13 修回日期:2013-11-10 出版日期:2014-04-25 发布日期:2014-05-06
  • 基金资助:

    国家自然科学基金项目(31270663);国家转基因生物新品种培育科技重大专项(2011ZX08009-003-002)。

Isolation and Expression Analysis of PwWDS1 in Picea wilsonii

Li Changjiang1, Cui Xiaoyan2, Sun Fan1, Zhang Lingyun1   

  1. 1. Key Laboratory of Forest Silviculture and Conservation of Ministry of Education College of Forestry, Beijing Forestry University Beijing 100083;
    2. College of Plant Science, Jilin University Changchun 130062
  • Received:2013-06-13 Revised:2013-11-10 Online:2014-04-25 Published:2014-05-06
  • Contact: 张凌云

关键词: 青杄, WDS, 生物信息学分析, 组织表达, 胁迫响应

Abstract:

Drought-induced proteins, which are induced or up-regulated by water deficient stress, play an important role in drought resistance process of plants. In this research, full length cDNA of PwWDS1 (GenBank accession number KJ526353), a drought induced gene, was obtained by RACE PCR method based on the full length cDNA library of Picea wilsonii. Several software and tools such as Expasy, DNAMAN, ClustalX and MEGA were used to analyze the physical and chemical properties of PwWDS1. Then expression pattern of PwWDS1 was identified by RT-qPCR and semi-quantitative RT-PCR under drought, salt stress, abscisic acid (ABA) and ethrel treatments. We found that the full length cDNA of PwWDS1 was 1 077 bp encoding a protein which consists of 207 amino acids residues. The theoretical molecular weight of PwWDS1 was 22.76 kDa with isoeletric point of 5.02. Sequence analysis indicated that C terminal of PwWDS1 had a conserved ABA/WDS domain and N terminal had a strong hydrophilic property. RT-qPCR and semi-quantitative RT-PCR showed that PwWDS1 was mainly expressed in needles of P. wilsonii and the expression level of PwWDS1 increased with the growth of cotyledon during seed germination. The PwWDS1 expression was up-regulated by drought, salt stress, ethrel and ABA treatments. PwWDS1 was expressed highly in 6 h under drought and ABA treatments. Under salt and ethrel treatments, PwWDS1 was expressed most in 12 h. These results indicated that PwWDS1 would play a role in response to signals of abiotic stresses and plant hormones in P. wilsonii.

Key words: Picea wilsonii, water deficiency stress(WDS), bioinformatic analysis, tissue expression, stress response

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