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林业科学 ›› 2014, Vol. 50 ›› Issue (4): 121-128.doi: 10.11707/j.1001-7488.20140418

• 研究简报 • 上一篇    下一篇

DHAP胁迫对天山云杉幼苗内源激素含量变化的影响

章建红1, 陈志颖2, 阮晓2, 张玉竹2, 潘存德3, 王强2   

  1. 1. 宁波农业科学院 宁波 315040;
    2. 浙江大学宁波理工学院 宁波 315100;
    3. 新疆农业大学林学与园艺学院 乌鲁木齐 830052
  • 收稿日期:2013-10-12 修回日期:2014-02-17 出版日期:2014-04-25 发布日期:2014-05-06
  • 基金资助:
    国家自然科学基金项目(30960313)。

Change of Endogenous Plant Hormones Contents during Seedling Growth of Picea schrenkiana Treated with DHAP

Zhang Jianhong1, Chen Zhiying2, Ruan Xiao2, Zhang Yuzhu2, Pan Cunde3, Wang Qiang2   

  1. 1. Ningbo Agriculture Academy Ningbo 315040;
    2. Ningbo Institute of Technology, Zhejiang University Ningbo 315100;
    3. College of Forestry and Horticulture, Xinjiang Agricultural University Urumqi 830052
  • Received:2013-10-12 Revised:2014-02-17 Online:2014-04-25 Published:2014-05-06
  • Contact: 王强

关键词: 天山云杉, 自毒物质, 超高效液相色谱(UPLC), 幼苗生长, 内源植物激素

Abstract: Schrenk spruce (Picea schrenkiana), a major tree species in the forest ecosystems of Xinjiang, plays an important role in water conservation. 3,4-dihydroxyacetophenone (DHAP) is a principal autotoxic substance in P. schrenkiana needles as well as its litter, and the substance may contribute to P. schrenkiana natural regeneration. In this paper, we determined the change of endogenous plant hormones (ZT、GA3、IAA and ABA) contents during seedling growth of P. schrenkiana with the ultra performance liquid chromatography (UPLC), and measured growth in radicle, plumule and fresh weight of the seedlings subjected to DHAP treatments, trying to explain physiological mechanisms of the autotoxic effects. The results showed that DHAP treatment had no significant effect on plumule growth of P. schrenkiana but had significant inhibitory effect on fresh weight (The fresh weight treated with 0.1 mmol·L-1 DHAP significantly increased in 10 d). The impact of DHAP treatment on radicle elongation had a concentration effect in an order of: 5 mmol·L-1>1 mmol·L-1>0.1 mmol·L-1>control (After 10 d, radicle elongation of 0.1 mmol·L-1 DHAP treatment groups increased significantly). With DHAP concentration increased, the GA3 concentration decreased, the ABA concentration increased, and the ratios of ABA/ZT、ABA/IAA、ABA/(ZT+GA3+IAA) increased. In 4-6 d, after 5 mmol·L-1 DHAP treatment, the IAA concentration increased greatly (1.24-1.70 times higher than that of control group), which might inhibit the seedling growth. For 0.1 mmol·L-1 DHAP treatment group, after 10 d, the concentration of ZT and IAA increased, the concentration of ABA decreased, and the ratios of ABA/ZT、ABA/IAA、ABA/(ZT+GA3+IAA) decreased, which might cause the radicle elongation and fresh weight increasing rapidly. It is suggested that the change of contents and the ratios of endogenous plant hormones during the seedling growth caused by DHAP treatment might be the key factor inhibiting (promoting) or delaying the seedling growth (radicle, plumule elongation and fresh weight) of P. schrenkiana.

Key words: Picea schrenkiana, DHAP, UPLC, seedling growth, endogenous plant hormones

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