关键词: 尾叶桉, TSRF 1 基因, 遗传转化, 广谱抗病性

Abstract: In this study, the hypocotyls germinated from the seeds of Eucalyptus urophylla U6 were used as explants, and the different plant growth substance combinations for the regeneration and conditions for genetic transformation of E. urophylla were optimized by an orthogonal test. The TSRF 1 gene was confirmed to integrate into the E. urophylla genome by PCR and Southern blotting. The transgenic E. urophylla markedly enhanced the disease resistance to Ralstonia solanacearum. The transgenic plants had stronger resistance to the leaf disease infested with Phytophthora. After inoculation with R. solanacearum, the activity of PPO, PAL and SOD significantly increased. The expression of TSRF 1 gene was increased after inoculation with diseases, detected by the method of Real-time PCR. These results suggested that expression of the TSRF 1 gene could improve broad-spectrum disease resistance in the transgenic E. urophylla.

Key words: Eucalyptus urophylla, TSRF 1, genetic transformation, broad-spectrum disease resistance