关键词: 根癌农杆菌, 淡紫拟青霉, G-418, 遗传转化

Abstract:

In this study, the research is conidia of Paecilomyces lilacinus, a pathogenic fungus of plant nematodes, was selected as the receptor. An efficient genetic transformation system of P.lilacinus was established by harboring G-418 resistance cassette as a selective marker to construct vector pBI -G3C-aphI based on Agrobacterium tumefaciens-mediated transformation. Using this system, P. lilacinus was successfully transformed with an efficiency of 1 000-2 400 transformants per 10⁶ spores. It indicated that T-DNA had been integrated into the genome of P.lilacinus by PCR analysis on the transformants, and the genetic character of transformants remained stable. The establishment of T-DNA insertional mutants library would provides a basis for further selecting pathogenic mutants, and understanding pathogenisis of P.lilacinus, which is of great significance to foster a better anti-nematode strain and develop effective biocontrol agents.

Key words: Agrobacterium tumefaciens, Paecilomyces lilacinus, G-418, transformation