关键词: 肉桂, 超低温保存, 离体胚, 含水量, 防冻剂

Abstract:

By taking different freezing method, thawing method and cryoprotectants as treatment factors, the cryopreservation of Cinnamomum cassia excised embryos with different moisture content was studied, and the dehydrogenase activity, the conductivity and α-amylase activity were measured and contrasted after cryopreserving with before. The results showed that the key of cryopreservation technology must be succeeded in reducing the harm of conservation materials during the desiccation and cryopreservation process, by taking the optimum magnitude and type of cryoprotectants, freezing and thawing method. The preservation effects adding the suitable antifreezes broadened the optimum cryopreserving moisture content level, and then enlarged the cryopreserved scale of moisture content. Cryopreserved without cryoprotectants, the suitable moisture content of the C. cassia excised embryos is 30%～40%. And taking quick freezing-quick thawing methods was better than others. Otherwise, the effect cryopreserved with cryoprotectants was much better than that without cryoprotectants, associated with mild freezing-quick thawing methods or quick freezing-mild thawing methods.

Key words: Cinnamomum cassia, cryopreservation, exercised embryos, moisture content, cryoprotectants