关键词: 银杏, 子叶, 愈伤组织, 超低温保存

Abstract:

Studies of cryopreservarion of callus of maidenhair tree (Gingko biloba L.) Cotyledon were conducted.The relative viability of cryopreserved materials was effected by both of cryoprotectant and freezing rate.It was also important to undergo pre-growth and that material for a while at freezing to some low temperature. By pre-culturing on MS adding high concentration of sorbitol or mannitol for two days-with the combinative cryoprotectant 10% Dimethyl sulfoxide(DMSO)+0.5 mol·L^-1 sorbitol-slow freezing at 1 ℃·min^-1-maintaining for 10 min at -15 ℃ and for 30 min at -35 ℃-directly plunging into LN-stored for 1 day-rapidly thawing at 40 ℃ water. The relative viability (TTC value) was beyond 60 %.The recovery growth of materials stored at ultra-temperature was observed.

Key words: Maidenhair tree, Cotyledon, Callus, Cryopreservation