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林业科学 ›› 2010, Vol. 46 ›› Issue (4): 49-57.doi: 10.11707/j.1001-7488.20100408

• 论文 • 上一篇    下一篇

枇杷属植物遗传多样性的ISSR分析

王永清1 付燕2 杨芩3 罗楠1 邓群仙1 严娟1 曾建国4 阮光伦5   

  1. 1.四川农业大学林学园艺学院 雅安 625014; 2. 黔东南民族职业技术学院植物组培中心 凯里 556000; 3. 凯里学院环境与生命科学学院 凯里 556011;4.成都市龙泉驿区农村发展局 成都 610100;5. 重庆市涪陵区果品办公室 重庆 408000
  • 收稿日期:2008-12-30 修回日期:1900-01-01 出版日期:2010-04-25 发布日期:2010-04-25

Genetic Diversity of Eriobotrya Analyzed by ISSR Markers

Wang Yongqing1,Fu Yan2,Yang Qin3,Luo Nan1,Deng Qunxian1,Yan Juan1,Zeng Jianguo4,Ruan Guanglun5   

  1. 1. College of Forestry and Horticulture, Sichuan Agricultural University Ya’an 625014; 2.Center for Plant Tissue Culture, Qiandongnan Nationality Professional Technology College Kaili 556000; 3.College of Environmental and Life Science, Kaili University Kaili 556011; 4.Bureau of Rural Area Development of Longquanyi, Chengdu Chengdu 610100; 5. Fruit Office of Fuling, Chongqing Chongqing 408000
  • Received:2008-12-30 Revised:1900-01-01 Online:2010-04-25 Published:2010-04-25

摘要:

采用ISSR技术对41份枇杷属植物材料进行分类和遗传多样性研究。从100条引物中筛选出20条引物共产生436条带,其中多态性带392条,占89.9%。平均有效等位基因数为1.3,平均Nei’s基因多样性指数为 0.208 5,平均Shannon信息指数为0.332 3,表明枇杷属植物中具有较丰富的遗传多样性。发现特异性条带33个,但并未发现春季开花或秋冬开花的特异标记,所用ISSR标记分析枇杷属植物的亲缘关系所得聚类结果表明,开花时期不能作为枇杷属植物的分类依据。相似系数0.722可将41份枇杷属植物分为野生类群和栽培类群,而栽培品种却不能按单一性状进行聚类。对枇杷属植物的分类方法进行讨论。

关键词: 枇杷属, ISSR, 分类, 遗传多样性

Abstract:

The genetic diversity of Eriobotrya was analyzed by ISSR markers. Twenty primers selected from 100 candidates were applied to the amplification which produced 436 bands. Of them 392 bands (89.9%) were polymorphic. The average effective number of alleles, Nei’s gene diversity and Shannon’s information index were 1.3, 0.208 5 and 0.332 3, respectively, suggesting that the accessions was comparatively great genetic diversity. There were 33 specific markers, but no specific markers for spring florescence or autumn-winter florescence were found. The spring florescence accessions could not be clustered to one group according to the dendrogram, suggesting that it might be inappropriate to use florescence as a criterion for the classification of Eriobotrya. The 41 loquat accessions could be divided into wild type and cultivated type with the similarity coefficient of 0.722, while the cultivated type could not be further classified according to a single trait. Based on the data, a discussion was made on the classification methods of Eriobotrya.

Key words: loquat(Eriobotrya), ISSR, genetic relationship, genetic diversity