杨树叶片数量性状相关联标记及其图谱定位研究

doi:10.11707/j.1001-7488.20000106

林业科学 ›› 2000, Vol. 36 ›› Issue (1): 33-40.doi: 10.11707/j.1001-7488.20000106

杨树叶片数量性状相关联标记及其图谱定位研究

苏晓华李金花陈伯望张绮纹张香华

中国林业科学研究院林业研究所,北京100091

收稿日期:1999-01-21 修回日期:1900-01-01 出版日期:2000-01-25 发布日期:2000-01-25

DETECTION AND IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH QUANTITATIVE TRAITS OF LEAF IN POPLAR

Su Xiaohua,Li Jinhua,Chen Bowang,Zhang Qiwen,Zhang Xianghua

The Research Institute of Forestry,CAF Beijing100091

Received:1999-01-21 Revised:1900-01-01 Online:2000-01-25 Published:2000-01-25

摘要/Abstract

摘要：

利用数量遗传学方法和分子标记相结合,对美洲黑杨与青杨杂交产生的F₂群体5个叶片数量性状进行了相关联标记及其图谱定位研究。用单因素方差分析检测出与叶长、叶宽、叶长/ 宽比、叶柄长和叶面积相关联的RAPD 标记分别为10 ,10 ,4 ,9 ,12 个;联合贡献率分别为66.23% ,61.82% ,32.86% ,59.67% ,81.79% 。用双因素方差分析检测出与互作QTLs 紧密相关联的标记位点共19对,其中与叶长、叶宽、叶长/ 宽比、叶柄长和叶面积显著相关联的标记分别有4 对,2 对,1 对,5 对,7 对。与这5 个性状相关联的标记多数集中分布于第4 ,12 ,15 和17 条连锁群上。

关键词: 杨树, 数量性状, 关联标记, 图谱定位

Abstract:

Both quantitative genetics method and molecular marker method were used to identify marker loci associated with 5 quantitative leaf traits in a F₂population from a cross of Populus deltoides Marsh. and P. cathayana Rehd.. Though analysis of variance (ANOVA) possesses a low resolution in mapping QTLs, it is an effective method for detecting QTLs. Single\|factor analysis of variance was conducted for each locus-trait combination to identify QTLs. The results revealed that 10,10,4,9 and 12 markers were significantly associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively, which accounted for 66.23%, 61.82%, 32.86%, 59.67% and 81.79% of the total phenotypic variation respectively. Two-factor analysis of variance showed that 4,2,1,5 and 7 significant interactions among these markers were associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively. Most of marker loci associated with these quantitative traits were on the 4th,12th,15th and 17th line of linkage groups.

Key words: Poplar, Quantitative traits, Associated markers, Identification

苏晓华李金花陈伯望张绮纹张香华. 杨树叶片数量性状相关联标记及其图谱定位研究[J]. 林业科学, 2000, 36(1): 33-40.

Su Xiaohua;Li Jinhua;Chen Bowang;Zhang Qiwen;Zhang Xianghua. DETECTION AND IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH QUANTITATIVE TRAITS OF LEAF IN POPLAR[J]. Scientia Silvae Sinicae, 2000, 36(1): 33-40.

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杨树叶片数量性状相关联标记及其图谱定位研究

DETECTION AND IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH QUANTITATIVE TRAITS OF LEAF IN POPLAR

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