• 论文及研究报告 •

### 杨树叶片数量性状相关联标记及其图谱定位研究

1. 中国林业科学研究院林业研究所,北京100091
• 收稿日期:1999-01-21 修回日期:1900-01-01 出版日期:2000-01-25 发布日期:2000-01-25

### DETECTION AND IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH QUANTITATIVE TRAITS OF LEAF IN POPLAR

Su Xiaohua,Li Jinhua,Chen Bowang,Zhang Qiwen,Zhang Xianghua

1. The Research Institute of Forestry,CAF Beijing100091
• Received:1999-01-21 Revised:1900-01-01 Online:2000-01-25 Published:2000-01-25

Abstract:

Both quantitative genetics method and molecular marker method were used to identify marker loci associated with 5 quantitative leaf traits in a F2population from a cross of Populus deltoides Marsh. and P. cathayana Rehd.. Though analysis of variance (ANOVA) possesses a low resolution in mapping QTLs, it is an effective method for detecting QTLs. Single\|factor analysis of variance was conducted for each locus-trait combination to identify QTLs. The results revealed that 10,10,4,9 and 12 markers were significantly associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively, which accounted for 66.23%, 61.82%, 32.86%, 59.67% and 81.79% of the total phenotypic variation respectively. Two-factor analysis of variance showed that 4,2,1,5 and 7 significant interactions among these markers were associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively. Most of marker loci associated with these quantitative traits were on the 4th,12th,15th and 17th line of linkage groups.