关键词: 银杏, 茎段, 组织培养, 正交试验

Abstract:

The tissue culture of the stem section in Ginkgo biloba carried out in the paper,using MS medium with the addition of different concentrations of activated carbon,6-BA and NAA.The orthogonal table L₉(3⁴)with the compound of three elements with three levels was selected.The various actions on the culture effect were analysed,such as different hormone,including activated carbon or not,and the regeneration ability of stem section in different growth period.The results showed that the MS medium with activated carbon 0.5% +6-BA1.0mg·L^-1+NAA0.1mg·L^-1 +NAA 0.1 mg·L^-1 was the best for the culture of stem section in Ginkgo biloba. In all elements,the positive effect of activated carbon was the most obvious.The proper time of sampling was in the middle and last ten days of April.

Key words: Ginkgo biloba, Stem section, Tissue culture(in vitro culture), Orthogonal experiment