不同致病力松材线虫对红松早期防御酶活性影响

doi:10.11707/j.1001-7488.20220802

摘要/Abstract

摘要：

目的: 研究不同株系松材线虫对红松致病力的差异, 探究感病红松早期防御酶活性变化与致病力之间的关系, 为红松松材线虫病防治提供参考。方法: 以4年生红松幼苗为研究对象, 人工接种辽宁清原株系(QH-1)、江苏南京株系(NM-1)与重庆株系(CM-1)松材线虫, 每株接种2000条, 并在接种1~7天分别对幼苗进行叶片取样, 测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)与过氧化物酶(POD)。在完成早期防御酶活性统计后, 持续对接种红松幼苗进行病害(针叶变色、嫩枝变化)观察, 分析各株系松材线虫致病力差异。结果: QH-1、NM-1和CM-1接种红松幼苗后, 上述4种防御酶活性变化趋势存在明显差异, 且接种QH-1处理组防御酶活性变化幅度大于其他线虫处理组。致病力统计结果表明, QH-1、NM-1与CM-1对红松均有致病力, 其中QH-1致病力最强, NM-1与CM-1无明显差异。防御酶活性变化幅度与致病力统计结果表明, 强致病力松材线虫导致红松幼苗防御酶活性变化幅度最大。结论: 4年生红松接种不同致病力松材线虫后, 发病早期防御酶活性变化存在差异, 且强致病力松材线虫株系导致接种红松防御酶活性变化幅度大于弱致病力松材线虫株系。

关键词: 松材线虫, 红松, 防御酶, 致病力

Abstract:

Objective: This study aims to explore the relationships between pathogenicity of different isolates of Bursaphelenchus xylophilus and the amplitude of defensive enzymes in the host plant, by investigating the pathogenicity of different isolates, and the activity changes of superoxide dismutase (SOD), catalase (CAT), phenylalanine ammonia-lyase (PAL), and polyphenol oxidation (POD) in Pinus koraiensis seedlings after inoculated with B. xylophilus. This study would provide reference for the prevention and control of the pine wood nematode disease. Method: Four-year-old P. koraiensis seedlings were inoculated separately with three different isolates (QH-1, NM-1 and CM-1) of B. xylophilus, and each plant was inoculated with 2 000 nematodes. The leaves were collected every day from next day to day 7 after inoculation, and used to analysis the activity change of SOD, CAT, PAL and POD in P. koraiensis seedlings. The pathogenicity and the external symptom were observed daily. In 35 days after the inoculation, the morbidity was recorded and nematodes were extracted from wilt seedlings, to analyze the difference in pathogenicity among QH-1, NM-1, and CM-1. Result: The activity change of SOD, CAT, PAL and POD in P. koraiensis seedlings inoculated with QH-1, NM-1, and CM-1 was obviously different, and the amplitude of enzyme activity change of the seedlings with QH-1 treatment was greater than that with NM-1 and CM-1 treatments. All three isolates of B. xylophilus were able to cause wilt to P. koraiensis seedlings, with QH-1 being more virulent than other two isolates. The strong virulent strains had greater change amplitudes of enzyme activity in inoculated seedlings. Conclusion: The virulence of B. xylophilus can be determined by measuring and analyzing the amplitude of enzyme activity change of infected P. Koraiensis seedlings The strong virulent strains cause greater changes in the amplitudes of enzyme activity in inoculated seedlings.

Key words: Bursaphelenchus xylophilus, Pinus koraiensis, defensive enzyme, virulence

中图分类号:

S763

曹业凡,王曦茁,汪来发,汪祥,徐明,苏胜荣,郭伟. 不同致病力松材线虫对红松早期防御酶活性影响[J]. 林业科学, 2022, 58(8): 10-17.

Yefan Cao,Xizhuo Wang,Laifa Wang,Xiang Wang,Ming Xu,Shengrong Su,Wei Guo. Effects of Different Virulence Isolates of Bursaphelenchus xylophilus on Aetivitres Early Enzymes in Pinus koraiensis[J]. Scientia Silvae Sinicae, 2022, 58(8): 10-17.

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