林业科学 ›› 2019, Vol. 55 ›› Issue (10): 1-9.doi: 10.11707/j.1001-7488.20191001
徐呈祥,马艳萍,旦书艳,邹燕,陈玉林
收稿日期:
2018-11-21
出版日期:
2019-10-25
发布日期:
2019-11-22
基金资助:
Chengxiang Xu,Yanping Ma,Shuyan Dan,Yan Zou,Yuling Chen
Received:
2018-11-21
Online:
2019-10-25
Published:
2019-11-22
Supported by:
摘要:
目的: 探索施加硅(Si)以提高檀香紫檀苗木抗寒性的矿质营养吸收与分配基础,为进一步Si营养提高苗木抗寒性的作用提供参考。方法: 以12月龄苗木为试材,测试分析2种施Si量栽培6个月的苗木体内8种矿质元素的含量变化,(-3±0.5)℃胁迫下细胞内H+和Ca2+浓度,质膜和类囊体膜H+-ATP酶、Ca2+-ATP酶活性及类囊体膜蛋白含量与磷脂酶D活性的响应。结果: 施Si苗木S、Cl元素相对含量降低,K元素相对含量显著降低,Mg、P、Fe元素相对含量升高,Si、Ca元素相对含量大幅升高;施Si苗木质膜H+-ATP酶、Ca2+-ATP酶活性显著高于对照,低温胁迫下降低幅度小,特别是叶片质膜H+-ATP酶;低温胁迫下,不施Si苗木类囊体膜蛋白质含量及H+-ATP酶、Ca2+-ATP酶活性下降幅度大、显著低于施Si苗木,同时,磷脂酶D(PLD)活性强、低温胁迫下显著升高。结论: 施Si改善檀香紫檀苗木对矿质元素的吸收、微域分布及平衡性,Si介导低温胁迫下苗木质膜和类囊体膜离子泵等生化组分及其功能的稳定性。
中图分类号:
徐呈祥,马艳萍,旦书艳,邹燕,陈玉林. 硅对檀香紫檀苗木矿质元素吸收、微域分布及离子泵活性的影响[J]. 林业科学, 2019, 55(10): 1-9.
Chengxiang Xu,Yanping Ma,Shuyan Dan,Yan Zou,Yuling Chen. Effects of Silicon on Mineral Element Absorption, Micro-distribution and Ion Pump Activity of Red Sandalwood Seedlings[J]. Scientia Silvae Sinicae, 2019, 55(10): 1-9.
表1
Si对檀香紫檀苗木根尖横切面细胞中Si等8种矿质元素相对含量的影响①"
细胞 Cell | 施Si量 The amount of Si applied/(mmol·kg-1) | 矿质元素相对含量Relative content of mineral elements (%) | |||||||
Mg | Si | P | S | Cl | K | Ca | Fe | ||
表皮细胞 Epidermal cell | 0 | 0.85cd | 1.63cd | 2.11c | 5.83ab | 4.51a | 65.31b | 16.60c | 3.15ab |
1.5 | 1.39bc | 9.30a | 3.31ab | 4.51b | 3.85abc | 51.01d | 21.45ab | 5.19a | |
皮层细胞 Cortical cell | 0 | 0.61d | 0.52de | 1.50c | 4.36b | 3.76abc | 79.35a | 10.67d | 1.22b |
1.5 | 1.53b | 1.94bc | 3.08b | 4.05b | 3.15bc | 68.07b | 14.14c | 2.06b | |
中柱细胞 Stelar cell | 0 | 1.55b | 0.18e | 3.07b | 7.32a | 4.45ab | 61.36bc | 19.73b | 2.43b |
1.5 | 2.97a | 3.03b | 4.23a | 4.11b | 3.07c | 52.04d | 25.53a | 4.82a |
表2
Si对檀香紫檀苗木叶片横切面细胞中Si等8种矿质元素相对含量的影响"
细胞 Cell | 施Si量 The amount of Si applied/(mmol·kg-1) | 矿质元素相对含量Relative content of mineral elements (%) | ||||||||
Mg | Si | P | S | Cl | K | Ca | Fe | |||
上表皮细胞Upper epidermal cell | 0 | 2.54b | 0.67e | 1.14de | 4.52c | 3.45ab | 60.04a | 24.75b | 2.89b | |
1.5 | 3.04b | 1.38bc | 1.74cd | 3.00c | 3.12ab | 44.09c | 38.57a | 5.08a | ||
下表皮细胞 Lowerepidermal cell | 0 | 0.26c | 0.48e | 0.98e | 6.98ab | 3.58ab | 63.04a | 23.24b | 1.45cd | |
1.5 | 1.21c | 1.70ab | 1.92bc | 5.13bc | 1.07d | 50.13b | 36.73a | 2.14bc | ||
栅栏组织细胞 Palisade tissue cell | 0 | 3.50b | 1.09cd | 1.03de | 8.22a | 3.89a | 60.82a | 21.33b | 0.33e | |
1.5 | 5.17a | 1.88a | 1.98bc | 4.94bc | 2.35bc | 42.26c | 39.33a | 1.14cd | ||
海绵组织细胞 Sponge tissue cell | 0 | 0.79c | 0.19e | 2.63ab | 4.86bc | 3.36ab | 63.54a | 24.40b | 0.26e | |
1.5 | 2.78b | 0.63de | 3.27a | 4.13c | 1.88cd | 50.06b | 34.96a | 2.33bc |
表3
Si对檀香紫檀苗木根尖中柱和叶片栅栏组织细胞中矿质元素平衡的影响"
细胞Cell | 施Si量 The amount of Si applied/ (mmol·kg-1) | 矿质元素相对含量比值Ratio of the relative content of mineral elements | ||||||||
Mg/K | Si/K | P/K | Ca/K | Fe/k | Mg/Ca | Si/Ca | P/Ca | Fe/Ca | ||
根尖中柱细胞 Rootoot tip stelar cell | 0 | 0.03c | 0.00d | 0.05b | 0.32c | 0.04b | 0.08c | 0.01c | 0.16a | 0.12b |
1.5 | 0.06b | 0.06a | 0.08a | 0.49b | 0.09a | 0.12b | 0.12a | 0.17a | 0.19a | |
栅栏组织细胞 Palisade tissue cell | 0 | 0.06b | 0.02c | 0.02c | 0.35c | 0.01c | 0.16a | 0.05b | 0.05b | 0.02c |
1.5 | 0.12a | 0.04b | 0.05b | 0.93a | 0.04b | 0.13b | 0.05b | 0.05b | 0.03c |
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