花椒麻味素(酰胺类)含量的常规检测

林业科学 ›› 2014, Vol. 50 ›› Issue (2): 121-126.

花椒麻味素(酰胺类)含量的常规检测

李菲菲¹, 李孟楼², 崔俊², 高锦明¹

1. 西北农林科技大学理学院杨凌 712100;
2. 西北农林科技大学林学院杨凌 712100

收稿日期:2013-05-07 修回日期:2013-10-19 出版日期:2014-02-25 发布日期:2014-03-11
基金资助:
国家863计划项目（2005AA001260）；陕西省科学技术研究发展计划项目（2012NY2-19）。

Conventional Determination of the Contents of Numbing Components (Acylamides) in Prickly Ash

Li Feifei¹, Li Menglou², Cui Jun², Gao Jinming¹

1. College of Science, Northwest A & F University Yangling 712100;
2. College of Forestry, Northwest A & F University Yangling 712100

Received:2013-05-07 Revised:2013-10-19 Online:2014-02-25 Published:2014-03-11
Contact: 李孟楼

摘要/Abstract

摘要：

花椒中的8种链状不饱和脂肪酸酰胺是花椒特有的麻味成分，建立花椒麻味素（总酰胺）含量的快速测定方法对评定花椒及其产品质量有实际意义。通过三水平四因素正交试验，确定总酰胺浸膏的最佳超声波辅助提取条件为50 ℃、花椒粉末样品：95%乙醇=1：10、提取2.5 h；经过高效液相色谱法与甲醛快速滴定法检测花椒总酰胺含量的比较研究，得出甲醛快速滴定法检测结果的校正系数为2.269 6（R²=0.978 1）。同时，应用甲醛快速滴定法检测来自不同产地14种花椒中的总酰胺含量，结果表明高山区红花椒的总酰胺含量>竹叶花椒>低山区红花椒。

关键词: 花椒, 麻味素, 酰胺, 超声波辅助提取, 甲醛滴定法

Abstract:

The unique numbing components in prickly ash (Zanthoxylum) consist of 8 different types of chained unsaturated fatty acid acylamides. It is meaningful to establish a rapid determination method of the numbing components contents (total acylamides) in prickly ash in evaluating the quality of prickly ash and its products. A three-levels and four-factors orthogonal experiment was conducted. The optimum ultrasonic-assisted extraction conditions for the total acylamides extract were in a 95% ethanol solvent with a 1:10 solid-liquid ratio at 50 ℃ for 2.5 h. Through a comparison study of the rapid formaldehyde titration method with HPLC approach for detecting the total acylamides contents in prickly ash, the adjusted coefficient of determination with the quick formaldehyde titration method was 2.269 6 (R²= 0.978 1). In addition, the rapid formaldehyde titration assay was used to detect the total acylamides contents of 14 types of prickly ash from different habitats. The results indicated that the Z.bungeanum, grown in alpine regions, had the highest total acylamides contents, followed by Z.armatum and then Z.bungeanum, found in low-relief terrain.

Key words: prickly ash, numbing components, acylamides, ultrasonic-assisted extraction, formaldehyde titration assay

中图分类号:

S713

李菲菲, 李孟楼, 崔俊, 高锦明. 花椒麻味素(酰胺类)含量的常规检测[J]. 林业科学, 2014, 50(2): 121-126.

Li Feifei, Li Menglou, Cui Jun, Gao Jinming. Conventional Determination of the Contents of Numbing Components (Acylamides) in Prickly Ash[J]. Scientia Silvae Sinicae, 2014, 50(2): 121-126.

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