橡胶树水通道蛋白基因HbPIP1和HbPIP2的功能鉴定及其表达分析

doi:10.11707/j.1001-7488.20140111

林业科学 ›› 2014, Vol. 50 ›› Issue (1): 69-75.doi: 10.11707/j.1001-7488.20140111

橡胶树水通道蛋白基因HbPIP1和HbPIP2的功能鉴定及其表达分析

王进¹, 安锋^{2 3}, 蔡秀清¹, 邹智², 张薇¹, 林位夫²

1. 海南大学农学院海口 570228;
2. 中国热带农业科学院橡胶研究所农业部儋州热带作物科学观测试验站儋州 571737;
3. 澳大利亚迪肯大学先进材料研究院维多利亚吉朗 3216

收稿日期:2013-03-27 修回日期:2013-05-22 出版日期:2014-01-25 发布日期:2014-01-25
基金资助:
国家自然科学基金项目（31100460）；国家天然橡胶产业技术体系（CARS34）；中央级公益性科研院所基本科研业务费专项资金（中国热带农业科学院院本级，1630022012023）资助项目。

Functional Characterization and Expression Analysis of Aquaporin Genes (HbPIP 1 and HbPIP 2) in Hevea brasiliensis

Wang Jin¹, An Feng^{2 3}, Cai Xiuqing¹, Zou Zhi², Zhang Wei¹, Lin Weifu²

1. College of Agriculture, Hainan University Haikou 570228;
2. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences Danzhou Investigation & Experiment Station of Tropical Crops of Ministry of Agriculture Danzhou 571737;
3. Institute for Frontier Materials, Deakin University Geelong, Victoria, 3216, Australia

Received:2013-03-27 Revised:2013-05-22 Online:2014-01-25 Published:2014-01-25
Contact: 林位夫

摘要/Abstract

摘要：

利用非洲爪蟾卵母细胞对橡胶树水通道蛋白基因HbPIP 1 和HbPIP 2 进行功能鉴定，发现HbPIP 1 具有快速水分传输功能而HbPIP 2 不具该功能。推测HbPIP 1 基因表达可能与乙烯利刺激橡胶树增产机制有关。因此，选择对乙烯利较敏感的橡胶树PR107品种，利用RT-qPCR 和 Western blot技术检测HbPIP 1 在转录和翻译水平的表达变化，探讨其与乙烯利刺激后橡胶树排胶体积、排胶时间、干含和总固形物变化的关系。结果表明：HbPIP 1 在树皮、胶乳和次生木质部中具有较高的表达量；胶乳中HbPIP 1 的表达量随乙烯利刺激时间延长呈上升趋势，而树皮中HbPIP 1 的表达量则呈降低的趋势；乙烯利通过调节HbPIP 1 的基因表达促进水分向乳管细胞的运输，从而降低胶乳干含和总固形物，延迟排胶时间，增加排胶体积和产量。

关键词: 橡胶树, 水通道蛋白, 功能鉴定, 表达分析, 组织特异性表达, 乙烯利刺激, 胶乳稀释

Abstract:

In this study, Xenopus laevis oocytes were used to test the functional characterization of our previously cloned HbPIP 1 and HbPIP 2 aquaporin genes from rubber trees (Hevea brasiliensis). The result showed that HbPIP 1 had the function of transmembrane water transport however HbPIP 2 has not. We therefore infer that HbPIP 1 is involved in the mechanism of Ethrel-induced latex promotion. An Ethrel sensitive rubber clone PR107 was selected as the plant material. The gene expression profiles of HbPIP 1 at both transcript level and translation level were examined by RT-qPCR and Western blot to investigate their link with the effect of Ethrel on latex flow duration, latex volume, dry rubber content (DRC) and total solids content (TSC). It was found that the expression of HbPIP 1 in latex, bark and xylem was higher than that in petioles and blades of various periods. The expression of HbPIP 1 in PR107 latex presented an increase trend with the Ethrel treatment duration, while it was down-regulated in the bark. This expression might facilitate the water transport into rubber laticifers and therefore can decrease latex DRC and TSC, prolong latex flow and enhance the latex yield.

Key words: Hevea brasiliensis, aquaporin, function characterization, expression analysis, tissue-specific expression, Ethrel stimulation, latex dilution

中图分类号:

S718.46

王进, 安锋, 蔡秀清, 邹智, 张薇, 林位夫. 橡胶树水通道蛋白基因HbPIP1和HbPIP2的功能鉴定及其表达分析[J]. 林业科学, 2014, 50(1): 69-75.

Wang Jin, An Feng, Cai Xiuqing, Zou Zhi, Zhang Wei, Lin Weifu. Functional Characterization and Expression Analysis of Aquaporin Genes (HbPIP 1 and HbPIP 2) in Hevea brasiliensis[J]. Scientia Silvae Sinicae, 2014, 50(1): 69-75.

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橡胶树水通道蛋白基因HbPIP1和HbPIP2的功能鉴定及其表达分析

Functional Characterization and Expression Analysis of Aquaporin Genes (HbPIP 1 and HbPIP 2) in Hevea brasiliensis

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