关键词: 牡丹, APETALA 2, 基因克隆, 表达分析

Abstract:

In this work, a full-length cDNA sequence of APETALA 2 gene was obtained from petals of Paeonia suffruticosa 'Zhaofen’ using RT-PCR and RACE, named PsAP 2 (GenBank accession No. HM167511). Sequence analysis indicated that PsAP 2 is 2 138 bp in full length, containing a 5'-untranslated region (5'-UTR) of 47 bp, a 3'-UTR of 557 bp, and an opening reading frame (ORF) of 1 533 bp encoding a 510 predicted amino acids,and belongs to AP 2 family. Sequence alignment and phylogenetic analysis revealed that PsAP 2 shared more than 70% homology with Vitis vinefera. Relative Real-Time PCR analysis indicated that PsAP 2 expressed in roots, stems, leaves and four floral organs. The expression in petals was the highest and the expression in leaves was the lowest.

Key words: tree peony, APETALA 2, gene cloning, expression analysis