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林业科学 ›› 2011, Vol. 47 ›› Issue (9): 50-56.doi: 10.11707/j.1001-7488.20110909

• 论文 • 上一篇    下一篇

牡丹PsAP 2 基因的克隆及表达

任磊, 王雁, 周琳, 彭镇华   

  1. 中国林业科学研究院林业研究所 国家林业局林木培育重点实验室 北京 100091
  • 收稿日期:2010-11-18 修回日期:2011-03-14 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 王雁

Isolation and Expression of PsAP 2 Gene in Tree Peony

Ren Lei, Wang Yan, Zhou Lin, Peng Zhenhua   

  1. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry, CAF Beijing 100091
  • Received:2010-11-18 Revised:2011-03-14 Online:2011-09-25 Published:2011-09-25

摘要:

以牡丹品种'赵粉’为试材,采用RT-PCR和RACE方法从花瓣中获得1个牡丹APETALA 2 基因cDNA全长,命名为PsAP 2 ,GenBank登录号为HM167511。序列分析结果表明: PsAP2全长2 138 bp,包含47 bp的5'非编码区、557 bp的3'非编码区和1个长度为1 533 bp编码510个氨基酸的开放阅读框。氨基酸序列分析显示该基因属于AP2家族。序列比对和系统进化分析表明,PsAP 2 与葡萄的亲缘关系最近,相似性达70%以上。相对荧光定量PCR分析表明,PsAP 2 在根、茎、叶和四轮花器官中均有表达。花瓣中的表达量最高,叶片中表达量最低。

关键词: 牡丹, APETALA 2, 基因克隆, 表达分析

Abstract:

In this work, a full-length cDNA sequence of APETALA 2 gene was obtained from petals of Paeonia suffruticosa 'Zhaofen’ using RT-PCR and RACE, named PsAP 2 (GenBank accession No. HM167511). Sequence analysis indicated that PsAP 2 is 2 138 bp in full length, containing a 5'-untranslated region (5'-UTR) of 47 bp, a 3'-UTR of 557 bp, and an opening reading frame (ORF) of 1 533 bp encoding a 510 predicted amino acids,and belongs to AP 2 family. Sequence alignment and phylogenetic analysis revealed that PsAP 2 shared more than 70% homology with Vitis vinefera. Relative Real-Time PCR analysis indicated that PsAP 2 expressed in roots, stems, leaves and four floral organs. The expression in petals was the highest and the expression in leaves was the lowest.

Key words: tree peony, APETALA 2, gene cloning, expression analysis

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