关键词: CCR, RNAi, 木质素, 遗传转化

Abstract:

Total DNA of ‘Nanlin 95’ poplar was isolated by CTAB method with adjustment, and the fourth extron fragment(340 bp) from CCR genome amplified by using PCR methods. The PCR products were subcloned into pUCCRNAi with the forward and reverse primers, and transferred into pBI121 expression vector. The expression vector was introduced into ‘Nanlin 95’ by Agrobacterium mediated transformation. Six transformants, which contain interference sequence of CCR, were obtained and approved by molecular identification.In comparison with the control, Klason lignin content of transgenic plants reduced 9.7 percent, holocellulose content increased by 3.14 percent, and the fiber aspect ratio remarkably increased. The transgenic plants are better material for pulp making.

Key words: CCR, RNA interference, lignin, genetic transformation