关键词: 猴头菌, ITS序列, 木质素降解酶系统, 锰过氧化物酶, 漆酶

Abstract:

Researches on ligninolytic enzymes of white-rot fungi could lay a foundation for exploring their degrading mechanism and expression and regulation mechanism of genes encoding for them. The study firstly observed and described the cultural characteristic of Hericium erinaceum strain CB1, result showed that CB1could produce some chlamydospores. Then, its ITS sequence was amplified (GenBank accession number: GU584100), and phylogenetic analyses was made based on the ITS sequences for 5 species including 17 different regional strains from Hericium, results showed that H. erinaceum strain CB1 and other strains from H. erinaceum were nearer in genetic distance and clustered together with them. After the classification status of H. erinaceum strain CB1 was fixed, its major ligninolytic enzymes were detected. The results indicated that H. erinaceum could produce MnP and laccase simultaneously, but no LiP excreted. The activities of MnP and laccase are regular. Mn²⁺ was proved to be the essential factor for H. erinaceum to produce MnP, but not for laccase. The highest MnP and laccase activity were reached when sawdust was added to LNAS culture solution with Mn²⁺ substrate which were 45.56 U·L^-1and 61.85 U·L^-1, respectively. It is the first time to report that the lignin degrading enzymes of H. erinaceum in China.

Key words: Hericium erinaceum, ITS sequences, ligninolytic enzymes, manganese peroxidase, laccase