利用RAPD分析大青杨天然群体的遗传结构

林业科学 ›› 1997, Vol. 33 ›› Issue (6): 504-512.

利用RAPD分析大青杨天然群体的遗传结构

苏晓华¹,张绮纹¹,郑先武¹,张香华¹,归复²

1. 中国林业科学研究院林业研究所北京 100091
2. 北京市林业局北京 100029

收稿日期:1996-08-14 出版日期:1997-11-25 发布日期:1997-11-25
基金资助:
本文内容属国家自然科学基金及劳动人事部非教育系统留学回国人员科技活动资助项目

GENETIC STRUCTURE IN POPULUS USSURIENSIS KOM. CONFIRMED BY RAPD MARKERS

Xiaohua Su¹,Qiwen Zhang¹,Xianwu Zheng¹,Xianghua Zhang¹,Fu Gui²

1. The Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091
2. Forestry Bureau of Beijing Beijing 100029

Received:1996-08-14 Online:1997-11-25 Published:1997-11-25
Supported by:
本文内容属国家自然科学基金及劳动人事部非教育系统留学回国人员科技活动资助项目

摘要/Abstract

摘要：

本文利用RAPD分子标记技术从DNA分子水平上探测了大青场(populus ussuriensis Kom.)天然群体的遗传结构和分化程度。结果得出:用14个随机寡核苷酸引物共产生180个扩增片段,扩增片断在211bp至1636bp之间。Shannon表型多样度(Ho)估测值在群体间变动范围为0.271至0.392,平均为0.310。对分子水平变异分为群体间和群体内两部分进行分析,群体间分量占总变异的62.3%,群体内只占37.7%。不同引物在群体内探测能力也各不相同, CH1-1引物探测多样度(Ho)最高(0.540),而2116引物最低(0.151)。

关键词: 大青杨, 天然群体, RAPD, 遗传结构

Abstract:

This paper described the population structure of P. ussuriensis Kom. The fourteen random primers chosen for analysis generated 180 bands that ranged in size from 211 bp to 1636bp. Estimates of Shannon's phenotypic diversity index ranged between 0.271 and 0.392, averaging 0.310. Analysis of molecular variance partitioned the RAPD variation into the among-and within-population components. The among-population components accounted for 62.3% of the variation and within-population accounted for only 37.7%. Estimates of Ho also varied among primers, with CH_1-1, detecting. Highest (0.540) and 2116 detecting the lowest (0.151) value Of diversity.

Key words: Populus, Natural population, RAPD, Genetic sbucture

苏晓华,张绮纹,郑先武,张香华,归复. 利用RAPD分析大青杨天然群体的遗传结构[J]. 林业科学, 1997, 33(6): 504-512.

Xiaohua Su,Qiwen Zhang,Xianwu Zheng,Xianghua Zhang,Fu Gui. GENETIC STRUCTURE IN POPULUS USSURIENSIS KOM. CONFIRMED BY RAPD MARKERS[J]. Scientia Silvae Sinicae, 1997, 33(6): 504-512.

图/表 9

图1

表1

表2

图图版Ⅰ

表3

表4

表5

表6

图2

参考文献 20

1	Cook, L. M.. Population Genetics, Chapman and Hall, 1976, London
2	Gottlieb L. D. . Electrophoretic evidence and plant population. Prog. in Phylochem., 1981. 1, 1- 46.
3	Lynch M. , Milligan B. G. . Analysis of population genetic structure with RAPD markers. Mol Ecol, 1994. 3, 91- 99. doi: 10.1111/j.1365-294X.1994.tb00109.x
4	Strauss S. H. , Hong Y. P. , Hipkins V. D. . High levels of population differentiation for mitochondrial DNA haplotypesin Pinus radiata, muricata and attenuata. Theor Appl Genet, 1993. 85, 605- 611.
5	Yeh F. C. , Chong D. K. X. , Yang R C. . RAPD variation within and among natural populations of Trembling aspen(Populus tremuloides Michx.) from Alberta. The Joumal of Heredity, 1995. 86 (6): 454- 459. doi: 10.1093/oxfordjournals.jhered.a111620
6	Hong Y. P. , Hipkins V. D. , Strauss SH . Chloroplast DNA diversity among trees, populations and species in the Califomia closed-cone pine (Pinus radiata, Pinusmiuricata and pinusattenuata). Genetics, 1993. 135, 1187- 1196.
7	Chalmers K. J. , Waugh R. , Sprent J. I. , et al. Detection of gentic variation between and within Populations of Glinidia sepion. and G. maculata using RAPD markers. Heredity, 1992. 69, 465- 472. doi: 10.1038/hdy.1992.151
8	Liu Z. , Fumier G R. . Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen. Theor Appl Genet, 1993. 87, 97- 105. doi: 10.1007/BF00223752
9	矶田圭哉. ,第105回,日本林学会大会, 1994, 697
10	Mosseler A. . Low levels of genetic diversity in red pine confirmed byrandom amplified polymorphic DNA markers. Canadian Joumal of Forest Resarch, 1992. 22 (9): 1332- 1337. doi: 10.1139/x92-177
11	Doyle J. J. , Doyle J. L. . A rapid DNA isolation procedure for small quanities of fresh leaf tissue. Phytochem. Bull., 1987. 19, 11015.
12	苏晓华, 等. 大青杨及其近缘种的遗传变异和系统关系研究. 林业科学, 1996. 32 (2): 118- 124.
13	Rohlf F. J.. NTSYS-PC numerical taxonomyand Multivariate analysis system. State University of New York Pr., 1992, New York
14	张绮纹, 等. 大青杨群体变异及其选择的研究. 林业科学, 1993. 29 (1): P57- 62.
15	Gehring J. L. , Linhart Y. B. . Population structure and genetic differentiation in native and introdued populations of Deschampsia Caespuosa(Poaceae) in the colorado alpine. Amer. J. Bot, 1992. 79, 1337- 1343. doi: 10.1002/j.1537-2197.1992.tb13742.x
16	Taylor D. R. , Aarssen L. W. . Competitive relationships among genotypes of three perennial grasses: Implications for species coesistence. Amer. Natur, 1990. 136, 105- 327.
17	Turkington R. , Harper J. L. . The growth, distnbution and neighbor relationships of Trifalium repens in a permanent pasture IV. Fine-scale biotic differentation. J Eco, 1979. 67, 245- 254.
18	庞广昌. 群体遗传多样性和数据分析. 林业科学, 1995. 31 (6): 543- 550.
19	Clegg M. T. . Measuring plant mating systems. Bioscience, 1980. 30, 814- 818. doi: 10.2307/1308373
20	Recent advance in molecular genetics of tree species. Dendrome, 1994, 1(2): 8-13

[1]	孟艺宏,徐刚标,卢孟柱,姜小龙,郭飞龙. 长柄双花木种群遗传结构及种群历史[J]. 林业科学, 2020, 56(7): 55-62.
[2]	包文泉, 乌云塔娜, 杜红岩, 李铁柱, 刘慧敏, 王淋, 白玉娥. 基于SSR标记的西藏光核桃群体遗传多样性和遗传结构分析[J]. 林业科学, 2018, 54(2): 30-41.
[3]	杨汉波, 张蕊, 王帮顺, 徐肇友, 陈焕伟, 周志春. 木荷优树无性系种质SSR标记的遗传多样性分析[J]. 林业科学, 2017, 53(5): 43-53.
[4]	李河, 李杨, 徐建平, 周国英. 海南省油茶及其他寄主植物果生刺盘孢菌群体遗传结构分析[J]. 林业科学, 2016, 52(10): 80-88.
[5]	李培, 阙青敏, 欧阳昆唏, 李俊成, 湛欣, 朱芹, 张俊杰, 邓小梅, 陈晓阳. 不同种源红椿SRAP标记的遗传多样性分析[J]. 林业科学, 2016, 52(1): 62-70.
[6]	柴宇博, 孙柏玲, 刘君良, 秦特夫, 储富祥. 近红外光谱快速预测乙酰化木材的增重率[J]. 林业科学, 2014, 50(9): 124-128.
[7]	李江伟, 杨琴军, 刘秀群, 季华, 张卓文. 台湾杉遗传多样性的ISSR分析[J]. 林业科学, 2014, 50(6): 61-66.
[8]	杨明秀, 宋瑞清. 中国10省金黄壳囊孢菌的菌落特征和遗传多样性[J]. 林业科学, 2014, 50(6): 160-166.
[9]	林玲, 王军辉, 罗建, 陈帅. 砂生槐天然群体种实性状的表型多样性[J]. 林业科学, 2014, 50(4): 137-143.
[10]	胡昳恒, 党萌, 张甜, 罗桂春, 夏海龙, 周惠娟, 呼东峰, 贺亮, 马振华, 赵鹏. 秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系——基于nrDNAITS序列分析[J]. 林业科学, 2014, 50(12): 47-55.
[11]	王文婷, 张东, 胡德夫, 初红军, 曹杰, 葛炎, 艾尔肯·吉力力, 李凯. 新疆普氏野马马胃蝇蛆病主要病原体黑腹胃蝇溯源[J]. 林业科学, 2014, 50(11): 90-95.
[12]	杨梅, 张敏, 师守国, 康永祥, 刘建军. 武当木兰种群遗传结构的ISSR分析[J]. 林业科学, 2014, 50(1): 76-81.
[13]	杨钤, 谢映平, 樊金华, 邵生富, 吴俊, 王彦士, 赵常胜, 张英伟. 日本松干蚧3个地理种群的遗传分化[J]. 林业科学, 2013, 49(12): 88-96.
[14]	徐斌, 张方秋, 潘文, 刘有成. 我国红锥天然群体的遗传多样性和遗传结构[J]. 林业科学, 2013, 49(10): 162-166.
[15]	刘华波;王哲;刘君;麻丽颖;王斯琪;唐岩;庞晓明. 燕山山脉西伯利亚杏的遗传多样性和遗传结构[J]. 林业科学, 2012, 48(8): 68-74.