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林业科学 ›› 1995, Vol. 31 ›› Issue (1): 1-7.

• 论文及研究报告 •    下一篇

小叶杨原生质体培养中的激素和氮含量变化*

程淑婉,王影,李林果   

  1. 南京林业大学 南京 210037
  • 收稿日期:1993-09-11 出版日期:1995-01-25 发布日期:1995-01-25

VARIATIONS OF HORMONES AND NITROGEN CONTENT IN PROTOPLAST CULTURE OF POPULUS SIMONII

Shuwan Cheng,Ying Wang,Linguo Li   

  1. Nanjing Forestry University NanJing 210037
  • Received:1993-09-11 Online:1995-01-25 Published:1995-01-25

摘要:

以小叶杨(Populus simonii carr.)原生质体培养的愈伤组织为供试材料,用高压液相色谱法(HPLC), 测定它们在四种不同分化培养基上四大类内源激素(IAA,GA3, CTK, ABA)的含量变化,并检定有关N的生化反应。结果表明,不同分化培养基上的愈伤组织中内源激素含量和种类差异很大。导致细胞分裂素(CTK)含量高,种类多以及细胞分裂素与生长素的比值〔CTK/IAA)高的处理,有利于愈伤组织分化; 但若愈伤组织中含有内源脱落酸(ABA),则抑制分化.测定结果还表明,小叶杨原生质体培养的愈伤组织分化过程中,其硝酸还原酶活力与氨基态N含量互为消长,分化频率高的愈伤组织中,硝酸还原酶活力低,而氨基态N含量高.

关键词: 小叶杨, 原生质体培养, 内源激素, 分化频率

Abstract:

In the protoplast culture of Populus simonii Carr. ,the variations in contents of IAA, GA3, CTK, ABA four kinds of endogenous hormones in the calli differentiated from four differentiation culture media were determined by the high pressure liquid chromatography method, and the biochemical reactions of nitrogen related to them were also tested. It was shown that there was a great change in the contents and kinds of the endogenous houmones among the calli differentiated from the different media. The treatment which resulted in the higher content and more kinds of CTK, and the higher ratio of CTK/IAA within the calli was favourable to the calli differentiation, whereas if the ABA was contented in calli, the differentiation of the calli was inhibited. During their differentiation process, a mutually exclusive relation between the activity of nitrate reductase(NR) and the amino-N content was found, in the calli with higher differentiation frequancy the NR activity was low, but the amino-N content was high.

Key words: Populus simonii carr., Protoplast cultures, Endogenous hormones, Differentistion frequency