关键词: 丝石竹, 发根农杆菌, 遗传转化, Ri T-DNA

Abstract: Young stems, leaves and callus of Gypsphila paniculata were inoculated separately with Agrobacterium rhizogenes strain LBA 9402. After 18 days from inoculation hairy roots were induced on the sites inoculated, which had not geotropism, grew luxurianlly and produceed branches on MS medium without any hormone. Calli were induced by exposing hairy root fragmants to MS medium supplemented with 2, 4-D 2mg/L and BAP 0. 5mg/L, which were subcultured for 7-8 weeks on MS medium containing 2,4-D 0.2mg/L, zeatin 2mg/L, and then transfered to hormone free MS medium for a regenerated plant. Agropine and mannopine were found out in the hairy roots, calli derived from hairy roots and the regenerated plants by means of electrophoresis examination, indicating that Ri T-DNA of Agrobacterium rhizogenes was conformed into nuclear gene group of G, paniculata.

Key words: G. paniculata, Agrobacterium rhizogenes, Genetic transformation, Ri T-DNA