林业科学 ›› 2023, Vol. 59 ›› Issue (2): 58-66.doi: 10.11707/j.1001-7488.LYKX20220348
收稿日期:
2022-05-19
出版日期:
2023-02-25
发布日期:
2023-04-27
通讯作者:
卫星
E-mail:1329694945@qq.com
基金资助:
Xin Cheng(),Chunze Wu,Qingyu Wei,Wei Li,Xing Wei*
Received:
2022-05-19
Online:
2023-02-25
Published:
2023-04-27
Contact:
Xing Wei
E-mail:1329694945@qq.com
摘要:
目的: 研究水曲柳接种丛枝菌根真菌后对干旱环境的生长和生理响应,为丛枝菌根真菌在干旱环境中的应用提供理论依据,也为水曲柳在干旱环境下的苗木培育及生产提供技术支撑。方法: 以水曲柳幼苗为供试植物,选取幼套球囊霉菌和摩西球囊霉菌为供试菌种,设置单接幼套球囊霉菌(Ge)、单接摩西球囊霉菌(Gm)和混合接种(Mix:Ge+Gm)3种接菌方式,以不接菌苗(CK)为对照组;在温室内采用盆栽控水的方式模拟自然干旱,设置轻度干旱LD(控水5天)、中度干旱MD(控水10天)和重度干旱SD(控水15天)3个处理,与全程正常供水NW进行比较;研究水曲柳接菌苗对不同水分条件的菌根侵染率、生长、渗透调节和抗氧化系统响应。结果: 1) 干旱胁迫下,接菌能够促进苗木生长,提高苗木质量;混合接菌比单一接菌更有助于提高苗木生长。混合接种处理具有更高的菌根侵染率,相关分析发现,菌根侵染率与苗高增量、地径增量、生物量增量、根长、根表面积、根系平均直径、可溶性蛋白(Sp)和脯氨酸(Pro)含量、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性显著正相关(P<0.05),与丙二醛(MDA)含量显著负相关(P<0.05)。2) 轻度胁迫时,混合接种处理的苗高增长量是CK的230.30%;混合接种处理的Sp含量分别是Ge、Gm和CK的1.1倍、1.3倍和1.5倍,Pro含量分别较Ge、Gm和CK增加25.12%、34.03%和68.30%。干旱胁迫下,接菌苗的MDA含量显著低于未接菌苗(P<0.05);POD、SOD和过氧化氢酶(CAT)活性显著高于未接菌苗(P<0.05)。结论: 干旱胁迫下,接菌促进幼苗的生长,增强幼苗的渗透调节能力和抗氧化系统调节能力。与单一接菌相比,混合接菌苗木具有更强的抗旱性。
中图分类号:
程鑫,吴纯泽,韦庆钰,李伟,卫星. 水曲柳丛枝菌根真菌接菌苗对干旱胁迫的生长和生理响应[J]. 林业科学, 2023, 59(2): 58-66.
Xin Cheng,Chunze Wu,Qingyu Wei,Wei Li,Xing Wei. Growth and Physiological Responses of Fraxinus mandshurica Seedlings Inoculated with Arbuscular Mycorrhizal Fungi to Drought Stress[J]. Scientia Silvae Sinicae, 2023, 59(2): 58-66.
表1
水曲柳接菌苗对干旱胁迫的根系生长响应①"
干旱胁迫处理 Stress treatment | 接种处理 Inoculation treatment | 根长 Root length/cm | 根表面积 Root surface area/cm2 | 根系平均直径 Average root diameter/mm |
NW | CK | 1001.99c | 127.05c | 0.2672c |
Ge | 1242.27b | 162.85a | 0.2800b | |
Gm | 1109.35c | 147.43b | 0.2701c | |
Mix | 1388.86a | 168.08a | 0.2901a | |
LD | CK | 728.98d | 85.28d | 0.2353d |
Ge | 893.16b | 127.88b | 0.2570b | |
Gm | 844.49c | 114.89c | 0.2445c | |
Mix | 947.07a | 137.92a | 0.2678a | |
MD | CK | 861.59d | 105.15d | 0.2409d |
Ge | 1036.08b | 135.39b | 0.2645b | |
Gm | 953.78c | 123.96c | 0.2507c | |
Mix | 1171.67a | 145.99a | 0.2789a | |
SD | CK | 901.30c | 113.10d | 0.2457c |
Ge | 1131.71b | 144.86b | 0.2669b | |
Gm | 1045.85c | 132.36c | 0.2549c | |
Mix | 1229.15a | 153.03a | 0.2844a |
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