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林业科学 ›› 2022, Vol. 58 ›› Issue (6): 88-94.doi: 10.11707/j.1001-7488.20220609

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美国白蛾对摩西球囊霉定殖银中杨的适应和生理响应

谭明涛1,姜礅1,武帅1,张杰1,刘磊1,赵佳齐1,孟昭军1,严善春1,2,*   

  1. 1. 东北林业大学林学院 森林生态系统可持续经营教育部重点实验室 哈尔滨 150040
    2. 黑龙江省外来林木病虫害监测与防控重点实验室 哈尔滨 150040
  • 收稿日期:2021-01-15 出版日期:2022-06-25 发布日期:2022-09-24
  • 通讯作者: 严善春
  • 基金资助:
    国家重点研发计划项目(2018YFC1200400)

Adaptation and Physiological Response of Hyphantria cunea (Lepidoptera: Arctiidae) to Populus alba × P. berolinensis Colonized by Glomus mosseae

Mingtao Tan1,Dun Jiang1,Shuai Wu1,Jie Zhang1,Lei Liu1,Jiaqi Zhao1,Zhaojun Meng1,Shanchun Yan1,2,*   

  1. 1. School of Forestry, Northeast Forestry University Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education Harbin 150040
    2. Key Laboratory of Alien Forest Pest Monitoring and Control of Heilongjiang Province Harbin 150040
  • Received:2021-01-15 Online:2022-06-25 Published:2022-09-24
  • Contact: Shanchun Yan

摘要:

目的: 探明美国白蛾对丛枝菌根真菌(AMF)定殖银中杨的适应和生理响应, 为揭示AMF在林木与植物昆虫之间的相互作用奠定基础,为利用AMF诱导增强林木化学防御提供理论依据。方法: 对银中杨扦插苗进行外源接种摩西球囊霉(GM),在GM侵染率达到最高时(90天),采叶饲喂美国白蛾幼虫,观察美国白蛾幼虫的生长发育、食物利用情况,分析其消化酶和解毒酶的活性变化。结果: 与对照相比,取食GM侵染的树叶后,3~6龄幼虫体质量均不同程度的低于CK,但差异不显著;2~5龄幼虫发育历期显著延长,且3龄和5龄幼虫相对生长率显著降低;美国白蛾5龄幼虫食物消耗率无显著差异,食物转化率和利用率被抑制;6龄幼虫食物消耗率、转化率和利用率均无显著差异。5龄幼虫脂肪酶活性显著增加,谷胱甘肽-S-转移酶(GSTs)活性被抑制,蛋白酶和淀粉酶、羧酸酯酶(CarE)活性与对照无显著差异。6龄幼虫脂肪酶、蛋白酶和淀粉酶、GSTs和CarE活性均显著高于对照。结论: 接种GM的银中杨对美国白蛾的生长发育和食物利用在短时间内有一定抑制作用,美国白蛾会通过提高消化酶和解毒酶活性来突破这种抑制作用,维持正常生长发育。

关键词: 美国白蛾, 丛枝菌根真菌, 摩西球囊酶, 生长发育, 食物利用, 消化酶, 解毒酶

Abstract:

Objective: This study aims to investigate the adaptation and physiological response of Hyphantria cunea (Lepidoptera: Arctiidae) to Populus alba × P. berolinensis colonized with arbuscular mycorrhizae fungi (AMF). Method: The cuttings of P. alba × P. berolinensis were inoculated with Glomus mosseae (GM), and when the GM infection rate reached the highest (90 days), the leaves were harvested and fed to the larvae of H. cunea. Result: After feeding on GM infected leaves of the popular, the body weight of 3rd-6th instar larvae was lower than that of the control, but the difference was not significant. The development duration of 2nd-5th instar larvae was significantly prolonged, and the relative growth rates of 3rd and 5th instar larvae were significantly decreased. There was no significant difference in food digestion rate of 5th instar larvae of H. cunea, but food conversion rate and utilization rate were inhibited. There was no significant difference in food consumption rate, conversion rate and utilization rate of 6th instar larvae. The activity of lipase in 5th instar larvae was significantly increased, the activity of glutathione S-transferase (GSTs) was inhibited, and the activities of protease, amylase and carboxylesterase (CarE) were not significantly different from those in the control group. The activities of lipase, protease, amylase, GSTs and CarE of 6th instar larvae were significantly higher than those of the control. Conclusion: The P. alba × P. berolinensis inoculated with GM has a certain inhibitory effect on the growth, development and food utilization of H. cunea in a short period of time, and H. cunea can break through this inhibition by increasing the activities of digestive enzymes and detoxification enzymes, and maintain normal growth and development. The results can lay a theoretical foundation for the future study of AMF-forest-herbivorous insect system.

Key words: Hyphantria cunea, arbuscular mycorrhizae fungi (AMF), Glomus mosseae, growth and development, food utilization, digestive enzyme, detoxification enzyme

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