7种非悬铃木方翅网蝽寄主植物叶片粗提物对其成虫的生物活性

doi:10.11707/j.1001-7488.20220716

摘要/Abstract

摘要：

目的: 研究水杉、枫香、构树、毛白杨、木瓜、鹅掌楸、白蜡等7种非悬铃木方翅网蝽的寄主植物叶片挥发物对其成虫具有显著引诱作用而未成为其寄主的原因，为配置非寄主植物或利用非寄主植物提取物防控悬铃木方翅网蝽提供依据。方法: 研磨、过滤获取这7种非寄主植物叶片的粗提物，以粗提物饲喂悬铃木方翅网蝽成虫，测定对其存活的影响。以粗提物原液和不同体积分数的稀释液涂抹悬铃木叶片，测定对悬铃木方翅网蝽成虫的忌避活性。结果: 7种非寄主植物叶片粗提物原液饲喂的悬铃木方翅网蝽成虫的生存状况显著低于对照(清水)(P＜0.05)，其半致死时间、99%致死时间和平均存活时间均极显著短于对照(P＜0.01)，其中木瓜尤为明显。7种非寄主植物叶片的粗提物原液对悬铃木方翅网蝽成虫均具有忌避效果，忌避率最高的为构树(74.84%)，其次是白蜡(70.74%)、枫香(53.90%)、木瓜(53.33%)、水杉(51.66%)、毛白杨(49.47%)、鹅掌楸(43.19%)。粗提物稀释后，忌避效果降低，但忌避效果并非随体积分数降低呈单向递减。结论: 嗅觉上有吸引作用的水杉、枫香、构树、毛白杨、木瓜、鹅掌楸、白蜡等7种植物叶片粗提物中含有对悬铃木方翅网蝽成虫有毒杀作用或(和)忌避作用的物质，可能是这些植物未能被悬铃木方翅网蝽利用的原因之一。7种植物中，构树提取物对悬铃木方翅网蝽的忌避作用最强，木瓜提取物对网蝽的毒杀作用最大, 可进一步研究二者在悬铃木方翅网蝽防控中的可能应用。

关键词: 悬铃木方翅网蝽, 非寄主植物, 生物活性, 生存影响, 忌避活性

Abstract:

Objective: The main purpose of this paper is to study the reasons that seven plants, including Metasequoia glyptostroboides, Liquidambar formosana, Broussonetia papyrifera, Populus tomentosa, Chaenomeles sinensis, Liriodendron chinense and Fraxinus chinensis do not become the hosts of Corythucha ciliata although the volatiles from the leaves of these seven plants have a significant attracting effect on C. ciliata adults, soas to provide a basis information for the use of non-host plants by tree species arrangement or their extracts to prevent and control C. ciliate. Method: The crude extracts from the leaves of the seven non host plants were obtained by grinding and filtration. The crude extracts were fed to the adults of C. ciliata to determine the impact on their survival. The crude extracts and dilutions of different volume fraction were applied to the leaves of Platanus acerifolia to determine the repellent activity against the adults of C. ciliata. Result: The survival status of C. ciliata adults fed with crude leaf extracts of 7 non-host plants was significantly lower than the control (water), and the median lethal time, 99% lethal time and average survival time were extremely significant shorter than those of C.ciliata adults treated with water, especially C. sinensis. The crude extracts from the leaves of the seven non-host plants all have repellent effects on C. ciliata adults. The highest repellent rate was B. papyrifera (74.84%), followed by F. chinensis (70.74%), L. formosana (53.90%), C. sinensis (53.33%), M. glyptostroboides (51.66%), P. tomentosa (49.47%) and L. chinense (43.19%). When the crude extracts were diluted, the repellent effects decreased, but the effects did not decrease unidirectionally with the decrease in volume fraction. Conclusion: That the crude extracts of these seven non-plant which have an attractive olfactory effect, contain substances that are toxic or and repellent to the adults of C. ciliata, may be one of the reasons why these plants are not used by C. ciliata. Among the seven plants, the extracts of B. papyrifera had the strongest repelling effect and the extracts of C. sinensis have the greatest toxicity to C. ciliata. The possible applications of the two plants in the control of C. ciliata can be studied further.

Key words: Corythucha ciliata, non-host plant, biological activity, survival impact, repellent activity

中图分类号:

S763

战鑫,庄敏,李国华,尹淑艳. 7种非悬铃木方翅网蝽寄主植物叶片粗提物对其成虫的生物活性[J]. 林业科学, 2022, 58(7): 156-162.

Xin Zhan,Min Zhuang,Guohua Li,Shuyan Yin. Biological Activity of Crude Extracts from Leaves of Seven Non-Host Plants against Corythucha ciliata Adults (Hemiptera: Tingidae)[J]. Scientia Silvae Sinicae, 2022, 58(7): 156-162.

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生存条件 Living conditions	半致死时间 Median lethal time/h	99%致死时间 99% lethal time/h	平均存活时间Average survival time/h
纯净水(对照)Pure water (control)	74.17±6.90Bb	131.32±15.94Bb	77.47±6.73Bb
悬铃木P. acerifolia	123.60±5.79Aa	218.65±9.24Aa	126.67±5.89Aa
水杉M. glyptostroboides	63.34±1.97BCc	107.35±7.57BCc	67.93±2.20BCbc
枫香L. formosana	53.45±1.42CDEcd	89.66±4.01Ccd	57.73±1.23CDEcd
构树B. papyrifera	39.12±1.30Ee	78.12±4.60Cd	43.07±1.14Ee
毛白杨P. tomentosa	46.67±2.82DEde	85.21±6.22Ccd	50.60±2.73DEde
木瓜C. sinensis	20.74±0.58Ff	33.99±0.31De	26.00±0.92Ff
鹅掌楸L. chinense	55.34±2.29CDcd	100.76±1.22BCcd	58.93±2.43CDcd
白蜡F. chinensis	44.99±2.62DEde	90.47±7.18Ccd	48.80±2.62DEde

植物 Plant	水杉 M. glyptostroboides	枫香 L. formosana	构树 B. papyrifera	毛白杨 P. tomentosa	木瓜 C. sinensis	鹅掌楸 L. chinense	白蜡 F. chinensis
忌避率 Repellent rate(%)	51.66±2.16ABbc	53.90±2.63ABbc	74.84±4.67Aa	49.47±3.68ABc	53.33±5.30ABbc	43.19±2.59Bc	70.74±9.81ABab

体积分数 Volume fraction	水杉 M. glyptostroboides	枫香 L. formosana	构树 B. papyrifera	毛白杨 P. tomentosa	木瓜 C. sinensis	鹅掌楸 L. chinense	白蜡 F. chinensis
100	51.66±2.16Aa	53.90±2.63Aa	74.84±4.67Aa	49.47±3.68Aa	53.33±5.30Aa	43.19±2.59Aa	70.74±9.81Aa
75	38.95±7.38ABa	42.04±1.32ABab	61.56±1.84ABa	32.68±4.35ABa	52.30±5.72Aa	38.65±2.03Aa	16.02±1.27Bb
50	19.38±9.78ABab	8.77±4.72Bc	29.32±4.76Cb	6.42±0.62BCb	21.79±5.58Ab	16.19±2.06Aab	26.91±0.83Bb
25	-10.21±8.51Bb	25.47±0.14ABbc	33.12±0.32BCb	-8.10±1.04Cb	17.29±2.92Ab	5.72±0.88Ab	23.11±0.69Bb

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