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林业科学 ›› 2007, Vol. 43 ›› Issue (09): 27-30.doi: 10.11707/j.1001-7488.20070906

• 论文及研究报告 • 上一篇    下一篇

地肤不同部位提取物对山楂叶螨活性的研究*

曹挥1,2 王有年1 张铁强1 李奕松1 刘素琪2 李向花2 师光禄1   

  1. 1.农业应用新技术北京市重点实验室 北京102206;2.山西农业大学农学院 太谷030801
  • 收稿日期:2006-02-28 修回日期:1900-01-01 出版日期:2007-09-25 发布日期:2007-09-25

Acarocidal Activity of Different Parts of Kochia scoparia against Tetranychus viennensis

Cao Hui1,2,Wang Younian1,Zhang Tieqiang1,Li Yisong1,Liu Suqi2,Li Xianghua2,Shi Guanglu1   

  1. 1.Key Lab.Of New Technology of Agricultural Application of Beijing Beijing 102206;2.College of Agriculture,Shanxi Agricultural University Taigu 030801
  • Received:2006-02-28 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

摘要:

研究地肤不同部位提取物对山楂叶螨的生物活性。结果表明:地肤子氯仿提取物的杀螨活性最高,浓度为2 mg·Ml-1对山楂叶螨的卵和雌成螨24 h后的校正死亡率分别达到63.37%和70.02%,明显高于根、茎和叶提取物。进一步萃取、柱层析分离发现:萃取物中氯仿相的活性明显大于水相、石油醚相和甲醇相,对氯仿萃取物的柱层析分离最终得到流分11个,其中以第2流分杀螨效果最好,浓度为l mg·Ml-1时,24 h对卵和雌成螨的校正死亡率分别达68.01%和87.10%。

关键词: 地肤, 山楂叶螨, 生物活性

Abstract:

The different parts of Kochia scoparia were investigated for their acarocidal activity against Tetranychus viennensis.The result indicated that the bioactivity of chloroform extract of seed of K.Scoparia was the higher than that of root,stem and foliage of K.Scoparia,the adjusted mortalities of egg and female mite were 63.37% and 70.02%.Respectively at the concentration of 2 mg·Ml-1 The chloroform extract of seed was then separated and purified.The silica gel column was used to separate and purify active constitutes of the crude extracts of chloroform.A total of 11 bands were seen by TLC analysis.The band 2 appeared a strong acarocidal activity against T.Viennensis,the adjusted mortalities of egg and female mite were 68.01% and 87.10%,respectively at the concentration of 1 mg·Ml-1

Key words: Kochia scoparia, Tetranychus viennensis, biological activity