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林业科学 ›› 2003, Vol. 39 ›› Issue (1): 98-102.doi: 10.11707/j.1001-7488.20030116

• 论文及研究报告 • 上一篇    下一篇

瑞香狼毒提取物对山楂叶螨的生物活性及酶活性影响

曹挥 刘素琪 赵莉蔺 师光禄 曾鑫年   

  1. 山西农业大学植保系,太谷030801;华南农业大学昆虫毒理室,广州510642
  • 收稿日期:2000-10-31 修回日期:1900-01-01 出版日期:2003-01-25 发布日期:2003-01-25

THE EFFECTS OF THE EXTRACTS FROM STELLERA CHAMAEJASME L.ON THE BIOLOGICAL AND ENZYME ACTIVITY OF TETRANYCHUS VIENNENSIS ZACHER

Cao Hui,Liu Suqi,Zhao Lilin,Shi Guanglu,Zeng Xinnian   

  1. Shanxi Agricultural University Taigu 03080;South China Agrcultural Univevsity\ Guangzhou 510642
  • Received:2000-10-31 Revised:1900-01-01 Online:2003-01-25 Published:2003-01-25

摘要:

瑞香狼毒的氯仿、石油醚、甲醇提取物对果树重要害螨山楂叶螨有很好的内吸和触杀活性;对氯仿提取物处理后的成螨体内几种重要酶系活性测定发现,在内吸处理中,其对蛋白酶、谷胱甘肽S 转移酶,酯酶同功酶有不同程度的激活作用;在触杀处理中,对蛋白酶、谷胱甘肽S 转移酶影响不明显,对酯酶同功酶有一定的抑制作用;研究表明,瑞香狼毒可以有效杀死山楂叶螨,是一种很有利用价值的杀虫植物。

关键词: 狼毒, 山楂叶螨, 生物活性, 酯酶同功酶, GSTs酶, 蛋白酶

Abstract:

The insecticidal activities of the four different extracts from a novel pesticidal plant—Stelera chamaejasme L. were studied. Three kinds of important enzyme systems of Tetranychus viennensis Zacher were tested. The results indicated that the extracts of S.chamaejasme had strongly pesticidal activities against T.viennensis. At the same time, when contacting with the extract in chloroform, the activities of protease, glutathione-S-transferase and esterase isozyme in T.viennensis were induced, but when feeding with the leaves absorbing the extract in chloroform, the activities of protease and glutathione-S-transferase in T.viennensis were not very clearly effected, but that of esterase isozyme was inhibited.

Key words: Stellera chamaejasme L., Tetranychus viennensis Zacher, Biological activity, Esterase isozyme, Glutathione-S-transferase, Protease