林业科学 ›› 2022, Vol. 58 ›› Issue (4): 82-94.doi: 10.11707/j.1001-7488.20220409
耿瑜欣1,李红姣1,郑建伟1,张芹2,于丽娜1,李佳秋1,李保会1,*
收稿日期:
2021-05-14
出版日期:
2022-04-25
发布日期:
2022-07-20
通讯作者:
李保会
基金资助:
Yuxin Geng1,Hongjiao Li1,Jianwei Zheng1,Qin Zhang2,Lina Yu1,Jiaqiu Li1,Baohui Li1,*
Received:
2021-05-14
Online:
2022-04-25
Published:
2022-07-20
Contact:
Baohui Li
摘要:
目的: 皂刺中的许多次生代谢物是药用活性成分。本文旨在探究皂荚与野皂荚刺的差异次生代谢物及进行差异代谢途径富集分析,为皂刺功能性化合物分离鉴定以及研究开发提供理论依据。方法: 以皂荚和野皂荚的1年生棘刺为材料,用UPLC-MS/MS检测平台和自建数据库MWDB4.0进行代谢组分析。结果: 1) 2个种的皂刺中共检测到457种次生代谢物,分为酚酸、生物碱、萜类、黄酮、木脂素、香豆素、鞣质类、其他8大类,酚酸和黄酮占比较大;2)野皂荚刺与皂荚刺相比,共有213种显著差异代谢物,其中121种物质在野皂荚刺中含量较高,包括55种酚酸,54种黄酮,5种木脂素和香豆素,4种生物碱,2种萜类,1种其他;92种物质在皂荚刺中含量较高,包括21种酚酸,19种黄酮,14种鞣质,14种生物碱,8种木脂素和香豆素,16种其他。3)皂荚刺中特有的代谢物有22种,异牡荆素-2″-O-鼠李糖苷、N,N′-二阿魏酰腐胺、香叶木素-8-C-(2″-O-鼠李糖基)葡萄糖苷、紫檀芪、原花青素C2相对含量较高,野皂荚刺中特有的代谢物有27种,松柏苷、5-O-咖啡酰莽草酸、槲皮素-3-O-(2″-对香豆酰)葡萄糖苷的相对含量较高。4)差异代谢物的代谢途径分析表明“茋类、二芳基庚烷类和姜酚的生物合成”途径中显著差异代谢物富集显著,苯丙烷生物合成和类黄酮代谢的差异代谢物富集较多。结论: 皂荚和野皂荚刺中共检测到457种次生代谢物;差异代谢物质主要是黄酮和酚酸类;茋类、二芳基庚烷类和姜酚的生物合成的差异代谢物显著富集(P < 0.05),苯丙烷生物合成和黄酮类生物合成的差异代谢物富集较多。
中图分类号:
耿瑜欣,李红姣,郑建伟,张芹,于丽娜,李佳秋,李保会. 皂荚与野皂荚刺中次生代谢物的差异[J]. 林业科学, 2022, 58(4): 82-94.
Yuxin Geng,Hongjiao Li,Jianwei Zheng,Qin Zhang,Lina Yu,Jiaqiu Li,Baohui Li. Difference of Secondary Metabolites in Spines of Gleditsia sinensis and Gleditsia microphylla[J]. Scientia Silvae Sinicae, 2022, 58(4): 82-94.
表1
皂荚和野皂荚刺差异表达代谢物"
序号 No. | 物质 Compounds | 分类 Classification | 差异倍数 log2FC |
1 | 松柏苷Coniferin | 酚酸Phenolic acid | 19.41 |
2 | 5-O-咖啡酰莽草酸5-O-Caffeoylshikimic acid | 酚酸Phenolic acid | 17.65 |
3 | 槲皮素-3-O-(2″-对香豆酰)葡萄糖苷Quercetin-3-O-(2″-p-coumaroyl)glucoside | 黄酮醇Flavonols | 17.62 |
4 | 1-羟基松脂醇-1-O-葡萄糖苷1-Hydroxypinoresinol-1-O-glucoside | 木脂素Lignans | 16.23 |
5 | 金圣草黄素-7-O-(6″-阿魏酰)葡萄糖苷Chrysoeriol-7-O-(6″-feruloyl)glucoside | 黄酮Flavonoid | 15.93 |
6 | 苜蓿酸-3-O-葡萄糖醛酸苷-28-O-鼠李糖基(1, 2)-阿拉伯糖苷 Medicagenic acid-3-O-glucuronide-28-O-rhamnosyl(1, 2)-arabinoside | 三萜皂苷 Triterpene saponin | 15.56 |
7 | 4-O-(6′-O-葡萄糖基咖啡酰)-4-羟基苯甲酸4-O-(6′-O-glucosylcaffeoyl)-4-hydroxybenzoic acid | 酚酸Phenolic acid | 15.18 |
8 | 咖啡酰对香豆酰酒石酸Caffeoyl-p-coumaroyltartaric acid | 酚酸Phenolic acid | 15 |
9 | 异杞柳苷-6″-O-对香豆酸Isosalipurposide-6″-O-p-coumaric acid | 查耳酮Chalcones | 14.33 |
10 | 3, 5-二咖啡酰奎宁酸3, 5-Dicaffeoylquinic acid | 酚酸Phenolic acid | 14.27 |
…… | …… | …… | …… |
204 | 白皮杉醇Piceatannol | 茋类Stilbene | -14.84 |
205 | 黄颜木素Fustin | 二氢黄酮醇Dihydroflavonol | -15.49 |
206 | 槟榔鞣质B1 Arecatannin B1 | 鞣质Tannin | -15.78 |
207 | 东莨菪内酯Scopoletin | 香豆素Coumarins | -16.33 |
208 | N-对香豆酰-N′-阿魏酰基腐胺N-p-coumaroyl-N′-feruloylputrescine | 酚胺Phenolamine | -16.71 |
209 | 原花青素C2 Procyanidin C2 | 原花青素Proanthocyanidins | -16.79 |
210 | 紫檀芪Pterostilbene | 茋类Stilbene | -17.34 |
211 | 香叶木素-8-C-(2″-O-鼠李糖基)葡萄糖苷Diosmetin-8-C-(2″-O-rhamnosyl)glucoside | 黄酮碳糖苷 Flavonoid carbonoside | -17.9 |
212 | N, N′-二阿魏酰腐胺N, N′-Diferuloylputrescine | 酚胺Phenolamine | -19.39 |
213 | 异牡荆素-2″-O-鼠李糖苷Isovitexin-2″-O-rhamnoside | 黄酮碳糖苷 Flavonoid carbonoside | -20.32 |
图6
皂荚和野皂荚刺相同代谢产物的相对含量 1:葫芦巴碱Trigonelline;2:对羟基苯甲酸4-hydroxybenzoic acid;3:香草醛Vanillin;4:柠檬酸*Citric acid*;5:异莨菪亭Isoscopoletin;6:奎宁酸Quinic acid;7:苯甲酰苹果酸Benzoylmalic acid;8:阿魏酰腐胺N-Feruloylputrescine;9:木犀草素Luteolin;10:香橙素Aromadendrin;11:高车前素*Hispidulin*;12:香叶木素*Diosmetin*;13:泽兰黄酮Nepetin;14:3-O-甲基槲皮素3-O-methylquercetin;15:5-O-对香豆酰奎宁酸5-O-p-coumaroylquinic acid;16:绿原酸*Chlorogenic acid*;17:东莨菪苷Scopolin;18:新绿原酸*Neochlorogenic acid*;19:阿魏酸-4-O-葡萄糖苷Ferulic acid-4-O-glucoside;20:3-O-阿魏酰奎宁酸3-O-feruloylquinic acid;21:异杞柳苷Isosalipurposide;22:樱桃苷Prunin;23:落新妇苷Astilbin;24:二氢山奈酚-3-O-葡萄糖苷Dihydrokaempferol-3-O-glucoside;25:香叶木素-7-O-半乳糖苷Diosmetin-7-O-galactoside;26:异槲皮苷*Isoquercitrin*;27:异鼠李素-3-O-葡萄糖苷*Isorhamnetin-3-O-glucoside*;28:蔓菁苷*Brassicin*;29:二咖啡酰基莽草酸Dicaffeoylshikimic acid;30:异绿原酸C Isochlorogenic acid C;31:异绿原酸B Isochlorogenic acid B;32:芹菜素-6-C-(2″-葡萄糖基)阿拉伯糖苷Apigenin-6-C-(2″-glucosyl)arabinoside;33:原花青素B1 Procyanidin B1;34:原花青素B3 Procyanidin B3;35:原花青素B2 Procyanidin B2;36:芦丁Rutin;37:槲皮素-3-O-芸香糖苷-7-O-葡萄糖苷Quercetin-3-O-rutinoside-7-O-glucoside;38:槲皮素-7-O-芸香糖苷-4′-O-葡萄糖苷Quercetin-7-O-rutinoside-4′-O-glucoside."
图7
皂荚刺特有代谢物的相对含量 1:东莨菪内酯Scopoletin;2:白皮杉醇Piceatannol;3:紫檀芪Pterostilbene;4:水杨苷Salicin;5:黄颜木素Fustin;6:芥子酰腐胺Sinapoylputrescine;7:3, 3′, 5-三羟基-4′, 7-二甲氧基黄烷酮3, 3′, 5-Trihydroxy-4′, 7-dimethoxyflavanone;8:罗汉松树脂醇Matairesinol;9:2, 4, 6, 4′-四羟基-二苯乙烯-2-O-葡萄糖苷2, 4, 6, 4′-tetrahydroxy-stilbene-2-O-glucoside;10:N-对香豆酰-N′-阿魏酰基腐胺N-p-coumaroyl-N′-feruloylputrescine;11: 对香豆酰阿魏酰腐胺p-coumaroylferuloylputrescine;12:N, N′-二阿魏酰腐胺N, N′-diferuloylputrescine;13:原花青素A1 Procyanidin A1;14:葛根素-4′3-葡萄糖苷Puerarin-4′3-glucoside;15:异牡荆素-2″-O-鼠李糖苷Isovitexin-2″-O-rhamnoside;16:原花青素A6Procyanidin A6;17:香叶木素-8-C-(2″3-鼠李糖基)葡萄糖苷Diosmetin-8-C-(2″-O-rhamnosyl)glucoside;18: 2′-乙酰毛蕊花糖苷2′-Acetylacteoside;19:肉桂单宁B1 Cinnamtannin B1;20:槟榔鞣质B1 Arecatannin B1;21:原花青素C2 Procyanidin C2;22:肉桂单宁B2 Cinnamtannin B2."
图8
野皂荚刺特有代谢物的相对含量 1:云杉苷Picein;2:柚皮素-4′, 7-二甲醚Naringenin-4′, 7-dimethyl ether;3:5-O-咖啡酰莽草酸5-O-caffeoylshikimic acid;4:松柏苷Coniferin;5:咖啡酰对羟基苯甲酰酒石酸Caffeoyl(p-hydroxybenzoyl)tartaric acid;6: 咖啡酰对香豆酰酒石酸Caffeoyl-p-coumaroyltartaric acid;7:6-O-葡萄糖基-阿魏酰苯甲酸6-O-glucosyl-feruloylbenzoic acid;8:4-O-(6′-O-葡萄糖基咖啡酰)-4-羟基苯甲酸4-O-(6′-O-glucosylcaffeoyl)-4-hydroxybenzoic acid;9:洋丁香苷A Syringalide A;10:4-对香豆酰鼠李糖基-(1→6)-D-葡萄糖4-p-cumaroyl-rhamnosyl-(1→6)-D-glucose;11:3, 4-二没食子酰莽草酸3, 4-digalloylshikimic acid;12:4-O-(6′-O-葡萄糖基咖啡酰)-3, 4-二羟基苯甲酸4-O-(6′-O-glucosylcaffeoyl)-3, 4-dihydroxybenzoic acid;13:4-O-(6′-O-葡萄糖基-阿魏酰)-3, 4-二羟基苄醇4-O-(6′-O-glucosylferuloyl)-3, 4-dihydroxybenzyl alcohol;14:1′-O-(3, 4-二羟基苯乙基)-O-咖啡酰基-葡萄糖苷1′-O-(3, 4-dihydroxyphenethyl)-O-caffeoyl-glucoside;15:阿魏酰阿魏酰酒石酸Feruloylferuloyltartaric acid;16:芥子酰对香豆酰酒石酸Sinapoyl-p-coumaroyltartaric acid;17:对香豆酰奎宁酸-4′-O-葡萄糖醛酸苷p-coumaroylquinic acid-4′-O-glucuronide;18:3, 5-二咖啡酰奎尼酸3, 5-dicaffeoylquinic acid;19:3-O-阿魏酰奎宁酸-O-葡萄糖苷3-O-feruloylquinic acid-O-glucoside;20:1-羟基松脂醇-1-O-葡萄糖苷1-hydroxypinoresinol-1-O-glucoside;21:5′-甲氧基罗汉松脂苷5′-methoxymatairesinoside;22:异杞柳苷-6″-O-对香豆酸Isosalipurposide-6″-O-p-coumaric acid;23:二芥子酰葡萄糖苷Disinapoyl glucoside;24:槲皮素-3-O-(2″-肉桂酰)葡萄糖苷Quercetin-3-O-(2″-cinnamoyl)glucoside;25:槲皮素-3-O-(2″-对香豆酰)葡萄糖苷Quercetin-3-O-(2″-p-Coumaroyl)glucoside;26:金圣草黄素-7-O-(6″-阿魏酰)葡萄糖苷Chrysoeriol-7-O-(6″-feruloyl)glucoside;27:苜蓿酸-3-O-葡萄糖醛酸苷-28-O-鼠李糖基(1, 2)-阿拉伯糖苷Medicagenic acid-3-O-glucuronide-28-O-rhamnosyl(1, 2)-arabinoside."
表2
皂荚和野皂荚刺差异代谢物KEGG通路"
序号 No. | 代谢通路(差异代谢物个数) KEGG pathway (quantity of differential metabolites) | P值 P-value |
1 | 茋类、二芳基庚烷类和姜酚的生物合成Stilbenoid, diarylheptanoid and gingerol biosynthesis(7) | 0.027 6 |
2 | 黄酮类生物合成Flavonoid biosynthesis(29) | 0.054 9 |
3 | 异黄酮生物合成Isoflavonoid biosynthesis(7) | 0.363 4 |
4 | 精氨酸和脯氨酸代谢Arginine and proline metabolism(5) | 0.383 2 |
5 | 叶酸生物合成Folate biosynthesis(1) | 0.437 9 |
6 | 硫胺代谢Thiamine metabolism(1) | 0.437 9 |
7 | 苯丙烷生物合成Phenylpropanoid biosynthesis(20) | 0.547 2 |
8 | 柠檬酸盐循环(TCA循环)Citrate cycle (TCA cycle)(4) | 0.591 1 |
9 | 泛醌和其他萜类-醌生物合成Ubiquinone and other terpenoid-quinone biosynthesis(4) | 0.591 1 |
10 | 乙醛酸和二羧酸代谢Glyoxylate and dicarboxylate metabolism(4) | 0.591 1 |
11 | 碳代谢Carbon metabolism(4) | 0.591 1 |
12 | 甘油磷脂代谢Glycerophospholipid metabolism(4) | 0.591 1 |
13 | 2-氧代羧酸代谢2-oxocarboxylic acid metabolism(4) | 0.591 1 |
14 | 次生代谢物的生物合成Biosynthesis of secondary metabolites(56) | 0.639 7 |
15 | 植物激素信号转导Plant hormone signal transduction(2) | 0.685 9 |
16 | 丙氨酸、天冬氨酸和谷氨酸代谢Alanine, aspartate and glutamate metabolism(2) | 0.685 9 |
17 | 花青素生物合成Anthocyanin biosynthesis(2) | 0.685 9 |
18 | 糖酵解/糖异生Glycolysis/gluconeogenesis(2) | 0.685 9 |
19 | 黄酮和黄酮醇的生物合成Flavone and flavonol biosynthesis(17) | 0.686 4 |
20 | 色氨酸代谢Tryptophan metabolism(11) | 0.796 3 |
21 | 苯丙氨酸、酪氨酸和色氨酸生物合成Phenylalanine, tyrosine and tryptophan biosynthesis(3) | 0.825 5 |
22 | 异喹啉生物碱生物合成Isoquinoline alkaloid biosynthesis(3) | 0.825 5 |
23 | 氨基酸的生物合成Biosynthesis of amino acids(6) | 0.826 9 |
24 | 烟酸和烟酰胺代谢Nicotinate and nicotinamide metabolism(4) | 0.903 6 |
25 | 各种次级代谢产物的生物合成-第2部分Biosynthesis of various secondary metabolites-part 2(6) | 0.971 1 |
26 | 苯丙氨酸代谢Phenylalanine metabolism(6) | 0.971 1 |
27 | 酪氨酸代谢Tyrosine metabolism(10) | 0.997 6 |
28 | 代谢途径Metabolic pathways(78) | 0.999 6 |
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