林业科学 ›› 2021, Vol. 57 ›› Issue (3): 126-134.doi: 10.11707/j.1001-7488.20210313
杨雨桐1,张卿硕2,符韵林2,孙静2,*
收稿日期:
2019-05-15
出版日期:
2021-03-01
发布日期:
2021-04-07
通讯作者:
孙静
基金资助:
Yutong Yang1,Qingshuo Zhang2,Yunlin Fu2,Jing Sun2,*
Received:
2019-05-15
Online:
2021-03-01
Published:
2021-04-07
Contact:
Jing Sun
摘要:
目的: 分析巴里黄檀心材色素成分,探究色素的稳定性和抗氧化性,为充分利用巴里黄檀资源提供理论基础和技术支撑,为同类红木资源的高效利用提供参考。方法: 从巴里黄檀木制品加工废料(刨花)中提取色素,使用超高效液相色谱-串联四级杆-静电场轨道阱高分辨质谱(UPLC-Q-EXACTIVE-MS)法分离鉴定色素成分,对色素主要成分进行定量;探究pH、金属离子、温度和紫外光照对色素稳定性的影响;以抗坏血酸(Vc)为阳性对照,分析色素对DPPH自由基和ABTS自由基的清除能力。结果: 从巴里黄檀心材色素中鉴定出荭草素、葛根素、牡荆素、异鼠李素、天竺葵素、矢车菊素、高车前素、白杨素、黄豆黄素、蓟黄素、樱花素、柚皮苷、染料木素、锦葵素、香叶木素、美迪紫檀素、芒柄花黄素、高丽槐素和6-羟基黄酮共19种黄酮类化合物,其中芒柄花黄素相对含量为2.27%;色素在酸性条件下稳定性较好,在碱性条件下稳定性差,pH>9后溶液出现沉淀;K+、Na+、Ba2+、Ca2+、Mg2+对色素几乎无影响,Al3+对色素有增色作用,Gr6+、Cu2+、Fe3+使色素产生沉淀,破坏作用较大;色素高温加热2 h内稳定性较好,长时间高温加热对色素有增色作用;紫外光照射对色素有缓慢增色作用;色素对DPPH自由基和ABTS自由基具有一定清除能力。结论: 巴里黄檀心材色素为黄酮类色素;色素在酸性至中性环境下较为稳定,在碱性条件下不稳定,Gr6+、Cu2+、Fe3+存在时不稳定,长时间高温加热和光照对色素稳定性影响较大;色素具有一定抗氧化能力。
中图分类号:
杨雨桐,张卿硕,符韵林,孙静. 巴里黄檀心材色素成分及其稳定性和抗氧化性[J]. 林业科学, 2021, 57(3): 126-134.
Yutong Yang,Qingshuo Zhang,Yunlin Fu,Jing Sun. Components, Stability and Antioxidant Activity of Pigment from Dalbergia bariensis Heartwood[J]. Scientia Silvae Sinicae, 2021, 57(3): 126-134.
表2
巴里黄檀心材色素中黄酮类成分离子结构信息"
峰号 No. | 保留时间 Retention time/min | 分子式 Formula | 一级质谱 MS(m/z) | 理论值 Theoretical value(m/z) | 二级质谱 MS2(m/z) | 化学成分 Component | 文献来源 Reference |
1 | 4.738 | C21H20O11 | 449.099 02 | [M+H]449.107 84 | 413.085 45 329.064 42 149.022 92 | 荭草素 Orientin | |
2 | 4.825 | C21H20O9 | 417.109 27 | [M+H]417.118 01 | 297.074 92 267.063 96 | 葛根素 Puerarin | |
3 | 5.094 | C21H20O10 | 433.104 27 | [M+H]433.112 92 | 397.090 55 313.069 58283.059 14 | 牡荆素 Vitexin | |
4 | 5.494 | C16H12O7 | 317.057 15 | [M+H]317.057 75 | 285.038 51 273.075 01149.022 90 | 异鼠李素 Isorhamnetin | |
5 | 6.109 | C15H10O5 | 271.051 83 | [M+H]271.052 27 | 271.059 11 | 天竺葵素 Pelargonidin | 胡莉等,2014 |
6 | 6.159 | C15H10O6 | 287.046 74 | [M+H]287.047 19 | 259.059 14 231.064 36149.022 96 | 矢车菊素 Cyanidin | 胡莉等,2014 |
7 | 6.205 | C16H12O6 | 301.062 24 | [M+H]301.062 89 | 301.069 70 245.079 86 167.033 36 | 高车前素 Hispidulin | |
8 | 6.522 | C15H10O4 | 255.056 89 | [M+H]255.057 36 | 255.064 32 229.085 13 | 白杨素 Chrysin | |
9 | 6.896 | C16H12O5 | 285.067 27 | [M+H]285.067 93 | 285.074 83 270.051 45 | 黄豆黄素 Glycitein | |
10 | 7.022 | C17H14O6 | 315.077 62 | [M+H]315.078 49 | 283.059 05 255.064 21 211.074 71 153.054 12 | 蓟黄素 Cirsimaritin | |
11 | 7.138 | C16H14O5 | 287.082 84 | [M+H]287.083 58 | 285.074 74 269.079 80 167.033 34 | 樱花素 Sakuranetin | |
12 | 7.243 | C27H32O14 | 579.175 86 | [M-H] 579.178 66 | 579.168 15 489.137 36 459.127 04 369.095 73 339.085 46 151.038 09 | 柚皮苷 Naringin | |
13 | 7.775 | C15H10O5 | 269.051 24 | [M-H] 269.052 27 | 269.065 12 254.057 63 | 染料木素 Genistein | |
14 | 7.867 | C17H14O7 | 331.072 57 | [M+H]331.073 40 | 331.080 14 316.056 73 271.059 20 | 锦葵素 Malvidin | 胡莉等,2014 |
15 | 8.14 | C16H12O6 | 301.062 24 | [M+H]301.062 84 | 284.031 19 256.036 19 228.077 18 151.037 99 | 香叶木素 Diosmetin | |
16 | 8.164 | C16H14O4 | 269.087 92 | [M-H] 269.088 66 | 269.043 79 254.056 67241.048 75 133.027 13 | 美迪紫檀素 Medicarpin | |
17 | 9.265 | C16H12O4 | 269.072 46 | [M+H]269.073 01 | 269.079 83 254.056 27 213.090 50 | 芒柄花黄素 Formononetin | |
18 | 9.474 | C16H12O5 | 285.067 28 | [M+H]285.067 93 | 151.038 54 123.043 85 | 高丽槐素 Maackiain | |
19 | 10.043 | C15H10O3 | 239.061 94 | [M+H]239.062 45 | 239.068 47 211.074 86193.064 03 165.069 46123.080 22 | 6-羟基黄酮 6-hydroxyflavone |
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