• 论文与研究报告 •

### 桂林喀斯特石山50种常见植物叶片光合色素含量及耐荫性定量评价

1. 1. 广西师范大学生命科学学院 桂林 541004;
2. 珍稀濒危动植物生态与环境保护教育部重点实验室 桂林 541004
• 收稿日期:2014-10-25 修回日期:2015-07-13 出版日期:2015-10-25 发布日期:2015-11-10
• 基金资助:
国家自然科学基金项目(31160156);广西自然科学基金项目(2014jjAA30037);广西高校科研项目(2013ZD008)。

### Leaf Photosynthetic Pigment Contents and Quantitative Evaluation of Shade Tolerance Among 50 Plant Species on Karst Rocky Mountain in Guilin

Ma Jiangming1,2, Huang Jing1, Yang Donglin1, Mei Junlin1

1. 1. College of Life Science, Guangxi Normal University Guilin 541004;
2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education Guilin 541004
• Received:2014-10-25 Revised:2015-07-13 Online:2015-10-25 Published:2015-11-10

Abstract: [Objective] In this study, quantitative characteristics of leaf photosynthetic pigments indexes of plant species in Karst rocky mountains in Guilin were analyzed to dig up the main shade tolerance indicators by using multivariate statistical method. Furthermore, the different plant species were classified based on the degree of shade tolerance to investigate the physiological characteristics of photosynthetic pigments by which the different plant species adapt their sites in the Karst rocky mountains. Thus, the study would provide a corresponding theoretical basis in restoring the degraded Karst rocky mountain habitat. [Method] A total of 50 common plant species growing in Karst rocky mountain of Guilin were selected to measure the leaf photosynthetic pigment indicators, including chlorophyll a (Chl a), chlorophyll b (Chl b), Carotenoid (Car), Chl (a+b) contents and Chl a/b. One-way ANOVA and post hoc Duncan test were used to test the significance of difference in each photosynthetic pigment indicator among the 50 plant species. Pearson correlation analysis was used to test the correlation between one photosynthetic pigment indicator and another. Principal component analysis (PCA) was used to find the main variables among the photosynthetic pigment indicators. The optimal partition of ordered sample method was used to divide the degree of shade tolerance among the 50 plant species based on the PCA ordination of shade tolerance. [Result] The average values of Chl a, Chl b, Car, Chl (a+b) and Chl a/b of the 50 plant species were 2.89 mg·dm-2,1.19 mg·dm-2,0.59 mg·dm-2,4.07 mg·dm-2, and 2.47, respectively. There were significant differences in all the 5 photosynthetic pigment indicators among the 50 plant species. There were significantly positive correlations among Chl a, Chl b, Car and Chl (a+b), and a negative correlation between Chl a/b and Chl b, and no significant correlation between Chl a/b and Chl a. The main differences of photosynthetic pigment indicators among the 50 plant species were the Chl (a+b) and chl a/b. The 50 plant species were divided into 3 types: type I with strong shade tolerance, type II with the characteristics combining shade plant and heliophyte, and type III with the characteristic of heliophyte or mesophyte. [Conclusion] The shade tolerance of the 50 plant species, which was decided based on the leaf chlorophyll content and Chl a/b ratio, was overall in accordance with their performance in the field, and the leaf chlorophyll contents and Chl a/b ratio were the important indicators which can reflect the shading resistance of plant. These research results would provide a reference on species selection in carrying out vegetation restoration and reconstruction in this area.