舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应

doi:10.11707/j.1001-7488.20140815

林业科学 ›› 2014, Vol. 50 ›› Issue (8): 102-107.doi: 10.11707/j.1001-7488.20140815

舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应

曹传旺, 孙丽丽, 问荣荣, 豆晓洁, 王志英

东北林业大学林学院哈尔滨 150040

收稿日期:2013-05-22 修回日期:2014-01-31 出版日期:2014-08-25 发布日期:2014-07-31
基金资助:
国家自然科学基金项目（31101676）；东北林业大学青年拔尖人才支持计划（PYTT-1213-10）；中央高校基本科研业务费专项基金（DL11BA02）。

Cloning and Analysis of LdOA1in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides

Cao Chuanwang, Sun Lili, Wen Rongrong, Dou Xiaojie, Wang Zhiying

College of Forestry, Northeast Forestry University Harbin 150040

Received:2013-05-22 Revised:2014-01-31 Online:2014-08-25 Published:2014-07-31
Contact: 王志英

摘要/Abstract

摘要：

G蛋白偶联受体（GPCRs）是生物体内重要膜受体，通过激活三聚体G蛋白参与各种细胞内信号转导，在药物研发中具有重要的作用。从舞毒蛾无参照全转录本文库中鉴定获得GPCR家族中眼白化I型全长基因（命名为LdOA1），该基因全长453 bp，编码150个氨基酸，分子质量为17.54 kDa，理论等电点（pI）为9.76。进化树分析表明LdOA1蛋白与帝王蝶的亲缘关系较近。实时荧光定量PCR分析表明舞毒蛾3龄幼虫在溴氰菊酯、甲萘威和氧化乐果胁迫下LdOA1的表达均表现为显著抑制。

关键词: 舞毒蛾, LdOA1基因, 杀虫剂, 基因表达

Abstract:

The G protein coupled receptors (GPCRs), known as transmembrane receptors, play an important role in drug research and development by activating G protein involved in inside signal transduction pathways. In present study, the full length cDNA of GPCR (namely LdOA1) was isolated from Lymantria dispar transcriptome. The open reading frame (ORF) of LdOA1 was 453 bp encoding a protein of 150 amino acid residues with the molecular mass of 17.54 kDa and theoretical pI of 9.76. Phylogenetic analysis of GPCR proteins showed LdOA1 of L. dispar clustered into a group with Danaus plexippus. We further investigated the expression of LdOA1 in 3^rd instar L. dispar larvae under deltamethrin, carbaryl and omethoate stresses using real-time PCR. The results showed that LdOA1 in L. dispar was obviously inhibited by deltamethrin, carbaryl and omethoate.

Key words: Lymantria dispar, LdOA1, insecticide, gene expression

中图分类号:

S718.7

曹传旺, 孙丽丽, 问荣荣, 豆晓洁, 王志英. 舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应[J]. 林业科学, 2014, 50(8): 102-107.

Cao Chuanwang, Sun Lili, Wen Rongrong, Dou Xiaojie, Wang Zhiying. Cloning and Analysis of LdOA1in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides[J]. Scientia Silvae Sinicae, 2014, 50(8): 102-107.

参考文献

谷学英, 刘志强, 刘玲, 等. 2009. 眼皮肤白化病Ⅰ型的基因诊断及产前诊断的意义.中国计划生育学杂志, 161(3): 168-171.
(Gu X Y, Liu Z Q, Liu L, et al. 2009. Significance of gene diagnosis and prenatal diagnosis for Oculocutaneous Albimism typeⅠ(OCA1). Chinese Journal of Family Planning, 161(3): 168-171. [in Chinese] )
侯雅芹, 南楠, 李镇宇. 2009. 舞毒蛾研究进展.河北林果研究, 24(4): 439-444.
(Hou Y Q, Nan N, Li Z Y. 2009. Research advances of Lymantria dispar. Hebei Journal of Forestry and Orchard Research, 24(4): 439-444. [in Chinese] )
倪鸣, 柴汝松, 张鹏, 等. 2009. 不同药剂对舞毒蛾和春尺蠖的毒力测定. 东北林业大学学报, 37(5): 121-122.
(Ni M, Chai R S, Zhang P, et al. 2009. Toxicity test of different kinds of insecticides to Lymantria dispar and Apocheima cinerarius. Journal of Northeast Forestry University, 37(5): 121-122. [in Chinese] )
Adams M D, Celniker S E, Holt R A, et al. 2000. The genome sequence of Drosophila melanogaster. Science, 287:2185-2195.
Brody T, Cravchik A. 2000. Drosophila melanogaster G Protein-coupled Receptors. The Journal of Cell Biology, 150: F83-F88.
Broeck J V. 2001. Insect G protein-coupled receptors and signal transduction. Archives of Insect Biochemistry and Physiology, 48:1-12.
d'Addio M, Pizzigoni A, Bassi M T, et al. 2000. Defective intracellular transport and processing of OA1 is a major cause of ocular albinism type 1. Human Molecular Genetics, 29 (20):3011-3018.
Hill C A, Fox A N, Pitts R J, et al. 2002. G protein-coupled receptors in Anopheles gambiae. Science, 298: 176-178.
Hill S J. 2006. G-protein-coupled receptors: past, present and future. British Journal of Pharmacology, 147: S27-S37.
Holt R A, Subramanian G M, Halpern A, et al. 2002. The Genome sequence of the malaria mosquito Anopheles gambiae. Science, 298:129-149.
Hu X B, Sun Y, Wang W J, et al. 2007. Cloning and characterization of NYD-OP7, a novel deltamethrin resistance associated gene from Culex pipiens pallens. Pesticide Biochemistry and Physiology, 88: 82-91.
Klabunde T, Hessler G. 2002. Drug design strategies for targeting G-protein-coupled receptors. ChemBioChem, 3: 928-944.
Lazarevi J, Peri-mataruga V, Ivanovi J, et al. 1998. Host plant effects on the genetic variation and correlations in the individual performance of the gypsy moth. Functional Ecology 12: 141-148.
Mitri C, Soustelle L, Framery B, et al. 2009. Plant insecticide L-canavanine repels Drosophila via the insect orphan GPCR Dmx. PLos Biology, 7(6): e1000147. doi:10.1371/journal.pbio.1000147.
Pfaffl M W, Horgan G W, Dempfle L. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30:36.
Schiaffino MV, d'Addio M, Alloni A, et al. 1999. Ocular albinism: evidence for a defect in an intracellular signal transduction system. Nature Genetics, 23(1): 108-112.
Verlinden H, Vleugels R, Marchal E, et al. 2010. The role of octopamine in locusts and other insects. Journal of Insect Physiology, 56:854-867.
Wittkopp P J, Williams B L, Carroll S B, et al. 2003. Drosophila pigmentation evolution: divergent genotypes underlying convergent phenotypes. Proceedings of the National Academy of Sciences of the USA, 100:1808-1813.
Zhou C, Rao Y, Rao Y. 2008. A subset of octopaminergic neurons are important for Drosophila aggression. Nature Neuroscience, 11 (9): 1059-1067.

[1]	刘道凤, 王霞, 代银, 杨建峰, 马婧, 李名扬, 眭顺照. 蜡梅转录因子CpTAF10基因的克隆及功能分析[J]. 林业科学, 2019, 55(6): 176-183.
[2]	卢惠君, 李子义, 梁瀚予, 岳远志, 周天畅, 杨玉璋, 王玉成, 及晓宇. 刚毛柽柳NAC24基因的表达及抗逆功能分析[J]. 林业科学, 2019, 55(3): 54-63.
[3]	范伟健, 相伟芳, 王静, 白鹏华, 潘丽娜, 杨艺新, 朱耿平, 李敏. 农药与紫外胁迫对白蛾周氏啮小蜂小分子热激蛋白sHSP12.2基因表达的影响[J]. 林业科学, 2019, 55(2): 128-136.
[4]	张扬, 杜琳, 唐贤丰, 刘唤唤, 周功克, 柴国华. 杨树BAG基因的鉴定及表达模式分析[J]. 林业科学, 2019, 55(1): 138-145.
[5]	魏明科, 俞金健, 黄晓龙, 刘琼瑶, 黄华宏, 林二培, 童再康. 杉木NAC转录因子基因ClNAC1的克隆、表达及单核苷酸多态性分析[J]. 林业科学, 2018, 54(9): 49-59.
[6]	王娟, 陈辉. 华山松大小蠹冷休克结合蛋白的鉴定与表达[J]. 林业科学, 2018, 54(4): 58-66.
[7]	张鹤华, 刘嘉欣, 罗朝兵, 张凌云. 青杄转录因子PwERF8及其启动子序列的克隆与分析[J]. 林业科学, 2018, 54(3): 48-60.
[8]	李建波, 贾会霞, 张进, 刘伯斌, 胡建军, 王丽娟, 卢孟柱. 毛白杨PtoWOX4a基因过表达对次生生长的影响[J]. 林业科学, 2018, 54(2): 52-59.
[9]	苑冉, 李欣悦, 吴韶平, 曹传旺. CO₂浓度升高对舞毒蛾生长发育和体内解毒酶及保护酶活性的影响[J]. 林业科学, 2018, 54(12): 102-109.
[10]	曾健勇, 张方明, 吴玥, 张婷婷, 张国财. 阿维菌素与杀铃脲对舞毒蛾幼虫的联合作用机制[J]. 林业科学, 2018, 54(12): 110-115.
[11]	姜礅, 薛羿, 徐智文, 王嘉冰, 孟昭军, 严善春. 喷施茉莉酸诱导长白落叶松抗性对舞毒蛾生长发育的影响[J]. 林业科学, 2018, 54(1): 162-167.
[12]	孙化雨, 李利超, 赵韩生, 杨意宏, 王思宁, 高志民. 黄藤2个NAC基因的分子特征及其SSR分子标记开发[J]. 林业科学, 2017, 53(8): 132-140.
[13]	唐绯绯, 赵玉琳, 王培龙, 冯德明, 宋怡, 高彩球. 刚毛柽柳ThP5CR基因的克隆及抗逆功能分析[J]. 林业科学, 2017, 53(7): 1-9.
[14]	李楠, 郑勇奇, 丁红梅, 柳新红, 盛炜彤, 江波, 李海波. 低温胁迫下短枝木麻黄耐寒相关基因的差异表达分析[J]. 林业科学, 2017, 53(7): 62-71.
[15]	党英侨, 殷晶晶, 陈传佳, 孙丽丽, 刘鹏, 曹传旺. 转舞毒蛾LdCYP6AN15v1基因果蝇品系对氯虫苯甲酰胺胁迫响应[J]. 林业科学, 2017, 53(6): 94-104.

舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应

Cloning and Analysis of LdOA1in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式