施氮对樟树林土壤微生物碳源代谢的影响

doi:10.11707/j.1001-7488.20140812

摘要/Abstract

摘要：

以亚热带地区樟树林为研究对象，设置3个氮添加水平，即0（对照）、5（低氮）和15 g·m^-2（高氮），采用BIOLOG—AN，FF，Gen Ⅲ和YT 4种微孔板，对施氮1年后土壤微生物群落碳源利用进行研究。结果表明：1）施氮后土壤微生物群落代谢活性均不同程度地降低，而且氮添加水平越高，土壤微生物代谢活性越弱；2）施氮可影响微生物群落多样性，施氮后，4种微生物群落丰富度增强，均匀度降低，厌氧菌、真菌和革兰氏菌的优势度降低；3）碳源利用分析表明，施氮后，糖类虽然仍是4种微生物群落主要利用的碳源，却相应地提高了其他几类碳源的利用程度，土壤真菌和厌氧菌的碳源利用受氮添加影响较大，而革兰氏菌和酵母菌碳源利用受氮添加影响相对较小。

关键词: 土壤微生物, 施氮, 碳源利用, 多样性, 樟树林

Abstract:

Human activities are greatly changing the way and rate of nitrogen input to the land ecological system on a global scale,which has a significant impact on the structure and function of soil ecosystem. To clarify the carbon source utilization of soil microbial communities in response to nitrogen addition, a field experiment was conducted in a Cinnamomum camphora plantation in Hunan Forest Botanical Garden. Three levels of nitrogen addition were applied, such as control (0 g·m^-2), low nitrogen (5 g·m^-2) and high nitrogen (15 g·m^-2). Using the BIOLOG AN,FF,Gen Ⅲ and YT microplate methods, carbon source utilization of soil microbial communities (anaerobic bacteria, fungi, gram bacteria and yeast) was investigated after 1 year treatment under different nitrogen additions. The results showed:1)Metabolic activity of soil microbial communities with nitrogen additions reduced to different degrees,and the higher the nitrogen concentration,the weaker soil microbial metabolic activities.2)Diversity index analysis showed that nitrogen inputs affected the diversity of four microbial communites. The richness of the four soil microbial communities increased, but the evenness reduced, and the dominance of the anaerobic bacteria, fungi and gram bacteria reduced under nitrogen additions.3)The analysis of carbon source utilization showed that the utilization of other carbon sources had increased although the carbonhydrates were still the main carbon resources of microbe utilization after nitrogen inputs. Principal component analysis showed that different nitrogen additions had a great impact on the soil microbial communities. The carbon sources utilization of soil fungi and anaerobic bacteria was greatly influenced by nitrogen input, but the effects of gram bacteria and yeast were relatively weakly by the influence of nitrogen input.

Key words: soil microbial, nitrogen addition, carbon source utilization, diversity, Cinnamomum camphora plantation

中图分类号:

S714.3

朱凡, 李天平, 郁培义, 宿少锋, 洪湘琦, 陈婷. 施氮对樟树林土壤微生物碳源代谢的影响[J]. 林业科学, 2014, 50(8): 82-89.

Zhu Fan, Li Tianping, Yu Peiyi, Su Shaofeng, Hong Xiangqi, Chen Ting. Carbon Source Utilization of Soil Microbial Communities in Response to Nitrogen Addition in the Cinnamomum camphora Plantation[J]. Scientia Silvae Sinicae, 2014, 50(8): 82-89.

参考文献

毕捷, 张乃莉, 梁宇, 等. 2012. 施氮和增雨对内蒙古半干旱地区草原土壤微生物群落碳源利用潜力的影响. 中国生态农业学报, 20(12): 1586-1593.
(Bi J, Zhang N L, Liang Y, et al. 2012. Impacts of increased N use and precipitation on microbial C utilization potential in the semiarid grassland of Inner Mongolia. Chinese Journal of Eco-Agriculture, 20(12): 1586-1593. [in Chinese] )
董艳, 杨智仙, 董坤, 等. 2013. 施氮水平对蚕豆枯萎病和根际微生物代谢功能多样性的影响. 应用生态学报, 24(4): 1101-1108.
(Dong Y, Yang Z X, Dong K, et al. 2013. Effects of nitrogen application rate on faba bean fusarium wilt and rhizospheric microbial metabolic functional diversity. Chinese Journal of Applied Ecology, 24(4): 1101-1108. [in Chinese] )
焦晓丹, 吴凤芝. 2004. 土壤微生物多样性研究方法的进展. 土壤通报, 35(6): 789-792.
(Jiao X D, Wu F Z. 2004. Progress of the methods for studying soil microbial diversity. Chinese Journal of Soil Science, 35(6): 789-792. [in Chinese] )
稂小洛, 曹国良, 黄学敏. 2008. 中国区域氮氧化物排放清单. 环境与可持续发展, (6): 19-21.
李娟, 赵秉强, 李秀英, 等. 2008. 长期不同施肥制度下几种土壤微生物学特征变化. 植物生态学报, 32(4): 891-899.
(Li J, Zhao B Q, Li X Y, et al. 2008. Changes of soil microbial properties affected by different long-term fertilization regimes. Journal of Plant Ecology (Chinese Version), 32(4): 891-899. [in Chinese] )
刘明, 李忠佩, 张桃林. 2009. 不同利用方式下红壤微生物生物量及代谢功能多样性的变化. 土壤, 41 (5): 744-748.
(Liu M, Li Z P, Zhang T L. 2009. Changes of microbial biomass and functional diversity in red soil under different land use types. Soils, 41 (5): 744-748. [in Chinese] )
卢蒙. 2009. 氮输入对生态系统碳、氮循环的影响: 整合分析. 上海:复旦大学博士学位论文.
(Lu M. 2009. The effects of nitrogen additions on ecosystem carbon and nitrogen cycles: a meta-analysis. Shanghai: PhD thesis of Fudan University. [in Chinese] )
鲁顺保, 张艳杰, 陈成榕, 等. 2013. 基于BIOLOG指纹解析三种不同森林类型土壤细菌群落功能差异. 土壤学报, 50(3): 618-623.
(Lu S B, Zhang Y J, Chen C R, et al. 2013. Analysis of functional differences between soil bacterial communities in three different types of forest soils based on Biolog fingerprint. Acta Pedologica Sinica, 50(3): 618-623. [in Chinese] )
罗希茜, 郝晓晖, 陈涛, 等. 2009. 长期不同施肥对稻田土壤微生物群落功能多样性的影响. 生态学报, 29(2): 740-748.
(Luo X Q, Hao X H, Chen T, et al. 2009. Effects of long-term different fertilization on microbial community functional diversity in paddy soil. Acta Ecologica Sinica, 29(2): 740-748. [in Chinese] )
王芸, 欧阳志云, 郑华, 等. 2012. 中国亚热带典型天然次生林土壤微生物碳源代谢功能影响因素. 生态学报, 32(6): 1839-1845.
(Wang Y, Ouyang Z Y, Zheng H, et al. 2012. Relationships between carbon source utilization of soil microbial communities and environmental factors in natural secondary forest in subtropical area,China. Acta Ecologica Sinica, 32(6): 1839-1845. [in Chinese] )
吴则焰, 林文雄, 陈志芳, 等. 2013. 武夷山国家自然保护区不同植被类型土壤微生物群落特征. 应用生态学报, 24(8): 2301-2309.
(Wu Z Y, Lin W X, Chen Z F, et al. 2013. Characteristics of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, East China. Chinese Journal of Applied Ecology, 24(8): 2301-2309. [in Chinese] )
薛璟花, 莫江明, 李炯, 等. 2007. 土壤微生物数量对模拟氮沉降增加的早期响应. 广西植物, 27(2): 174-179, 202.
(Xue J H, Mo J M, Li J, et al. 2007. The short-term response of soil microorganism number to simulated nitrogen deposition. Guihaia, 27(2): 174-179, 202. [in Chinese] )
郁培义, 朱凡, 宿少锋, 等. 2013. 氮素添加对樟树林红壤微生物的影响. 环境科学, 34(8): 3231-3237.
(Yu P Y, Zhu F, Su S F, et al. 2013. Effects of nitrogen addition on red soil microbes in the Cinnamomum camphora plantation. Environmental Science, 34(8): 3231-3237. [in Chinese] )
袁颖红, 樊后保, 李辉信, 等. 2012. 模拟氮沉降对杉木人工林土壤微生物的影响. 林业科学, 48(9): 8-14.
(Yuan Y H, Fan H B, Li H X, et al. 2012. Effects of simulated nitrogen deposition on soil microorganism in a Chinese fir plantation. Scientia Silvae Sinicae, 48(9): 8-14. [in Chinese] )
郑琼, 崔晓阳, 邸雪颖, 等. 2012. 不同林火强度对大兴安岭偃松林土壤微生物功能多样性的影响. 林业科学, 48(5): 95-100.
(Zheng Q, Cui X Y, Di X Y, et al. 2012. Effects of different forest fire intensities on microbial community functional diversity in forest soil in Daxing’anling. Scientia Silvae Sinicae, 48(5): 95-100. [in Chinese] )
周德庆. 1993. 微生物学教程. 北京:高等教育出版社, 198-199.
钟文辉, 蔡祖聪. 2004. 土壤微生物多样性研究方法. 应用生态学报, 15(5): 899-904.
(Zhong W H, Cai Z C. 2004. Methods for studying soil microbial diversity. Chinese Journal of Applied Ecology, 15(5): 899-904. [in Chinese] )
Aber J D, McDowell W, Nadelhoffer K, et al. 1998. Nitrogen saturation in temperate forest ecosystems: hypotheses revisited. BioScience, 48(11): 921-934.
Adams A B, Harriso R B, Sletten R S, et al. 2005. Nitrogen-fertilization impacts on carbon sequestration and flux in managed coastal Douglas-fir stands of the Pacific Northwest. Forest Ecology and Management, 220(1/3): 313-325.
Baath E, Berg B, Lohm U, et al. 1980. Effects of experimental acidification and liming on soil organisms and decomposition in a Scots pine forest. Pedobiologia, 20(2): 85-100.
Bowden R D, Davidon E, Savage K, et al. 2004. Chronic nitrogen additions reduce total soil respiration and microbial respiration in temperate forest soils at the Harvard Forest. Forest Ecology and Management, 196(1): 43-56.
Dijkstra F A, Hobbie S E, Reich P B, et al. 2005. Divergent effects of elevated CO₂, N fertilization and plant diversity on soil C and N dynamics in a grassland field experiment. Plant and Soil, 272(1/2): 41-52.
Frey S D, Knoor M, Parrent J L, et al. 2004. Chronic nitrogen enrichment affects the structure and function of the soil microbial community in temperate hardwood and pine forests. Forest Ecology and Management, 196(1): 159-171.
Grandy A S, Strickland M S, Lauber C L, et al. 2009. The influence of microbial communities, management, and soil texture on soil organic matter chemistry. Geoderma, 150(3/4): 278-286.
Grayston S J, Wang S, Campbell C D, et al. 1998. Selective influence of plant species on microbial diversity in the rhizosphere. Soil Biology & Biochemistry, 30(3): 369-378.
Guo J H, Liu X J, Zhang Y, et al. 2010. Significant acidification in major Chinese croplands. Science, 327: 1008-1010.
Juliet P M, Lynne B, Randerson P F. 2002. Analysis of microbial community functional diversity using sole-carbon-source utilization profiles-a critique. FEMS Microbiology Ecology, 42(1): 1-14.
Lekberg Y, Schnoor T, Kjller R, et al. 2012. 454-sequencing reveals stochastic local reassembly and high disturbance tolerance within arbuscular mycorrhizal fungal communities. Journal of Ecology, 100(1): 151-160.
Liu Z F, Fu B J, Zheng X X, et al. 2010. Plant biomass, soil water content and soil N:P ratio regulating soil microbial functional diversity in a temperate steppe: A regional scale study. Soil Biology & Biochemistry, 42(3): 445-450.
Lü C Q, Tian H Q. 2007. Spatial and temporal patterns of nitrogen deposition in China: Synthesis of observational data. Journal of Geophysical Research: Atmosphere, 112(D22)S05.
Magill A H, Aber J D, Currie W S, et al. 2004. Ecosystem response to 15 years of chronic nitrogen additions at the Harvard Forest LTER, Massachusetts, USA. Forest Ecology and Management, 196(1): 7-28.
Paul J W, Beauchamp E G. 1996. Soil microbial biomass C, N mineralization and N uptake by corn in dairy cattle slurry and urea amended soils. Canadian Journal of Soil Science, 76(4): 469-472.
Rockström J, steffen W, Noone K, et al. 2009. A safe operating space for humanity. Nature, 461:472-475.
Rogers B F, Tate Ⅲ R L. 2001. Temporal analysis of the soil microbial community along a top sequence in Pineland soils. Soil Biology & Biochemistry, 33(10): 1389-1401.
Rousk J, Brookes P C, Baath E. 2011. Fungal and bacterial growth responses to N fertilization and pH in the 150-year 'Park Grass' UK grassland experiment. FEMS Microbiology Ecology, 76(1): 89-99.
Sager E P S, Hutchinson T C. 2005. The effects of UV-B, nitrogen fertilization, and springtime warming on sugar maple seedlings and the soil chemistry of two central Ontario forests. Canadian Journal of Forest Research, 35(10): 2432-2446.
Sayer E J, Wright S J, Tanner E V J, et al. 2012. Variable responses of lowland tropical forest nutrient status to fertilization and litter manipulation. Ecosystems, 15(3): 387-400.
Silver W L, Thompson A W, Reich A, et al. 2005. Nitrogen cycling in tropical plantation forests: potential controls on nitrogen retention. Ecological Applications, 15(5): 1604 -1614.
Thoms C, Gattinger A, Jacob M, et al. 2010. Direct and indirect effects of tree diversity drive soil microbial diversity in temperate deciduous forest. Soil Biology & Biochemistry, 42(9): 1558 -1565.
Treseder K K. 2008. Nitrogen additions and microbial biomass: a meta-analysis of ecosystem studies. Ecology Letters, 11(10): 1111-1120.
Yan W J, Mayorga E, Li X Y, et al. 2010. Increasing anthropogenic nitrogen inputs and riverine DIN exports from the Changjiang River basin under changing human pressures. Global Biogeochemical Cycles, 24(4): 1-14.
Zak J C, Willig M R, Moorhead D L, et al. 1994. Functional diversity of microbial communities: a quantitative approach. Soil Biology & Biochemistry, 26(9): 1101-1108.
Zhang N, Wan S Q, Li L H, et al. 2008. Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China. Plant and Soil, 311(1/2): 19-28.
Zhou X B, Zhang Y M, Downing A. 2012. Non-linear response of microbial activity across a gradient of nitrogen addition to a soil from the Gurbantunggut Desert, northwestern China. Soil Biology & Biochemistry, 47(4): 67-77.

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